一个控制水稻叶色白化转绿及多分蘖矮杆性状基因hw-1(t)的鉴定  

Characterizations of a Mutant Gene hw-1(t) for Green-revertible Albino,High Tillering and Dwarf in Rice(Oryza sativa L.)

作　　者：郭涛[1] 黄宣[1] 黄永相[1] 刘永柱[1] 张建国[1] 陈志强[1] 王慧[1] 

机构地区：[1]华南农业大学/国家植物航天育种工程技术研究中心,广东广州510642

出　　处：《作物学报》2012年第1期23-35,共13页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30771313);国家科技支撑计划项目(2008BAD97B02)资助

摘　　要：通过空间诱变从光身稻品种Francis的M2群体中发现1株叶色白化转绿、多分蘖矮秆突变体hfa-1。hfa-1在三叶期之前完全白化,随后转绿。白化转绿表型受生长发育和温度调控。亚细胞结构观察发现hfa-1叶绿体发育异常抑制叶绿素合成,造成光合效率降低,产生白化表型。hfa-1的多分蘖表型是由于高节位分蘖芽激活所致,初步鉴定与苗期叶片IAA(吲哚乙酸)含量无关。hfa-1的矮生性则由节间长度缩短所致,与苗期GA(赤霉素)的合成和信号传导无关。遗传分析表明hfa-1的白化转绿、多分蘖矮秆表型受单隐性核基因hw-1(t)控制。利用hfa-1与粳稻品种02428杂交获得的F2群体将hw-1(t)定位在水稻第4染色体长臂上两个InDel标记HW27和HW7间46.9kb的物理距离内,该区域有13个阅读框架,其中LOC_Os04g57320编码IMMUTANTS蛋白,推测为hw-1(t)的候选基因。A rice（Oryza sativa L.） mutant hfa-1 exhibiting green-revertible albino,high-tillering dwarf was detected from a M2 population of an American variety Francis by space mutagenesis.hfa-1 displayed distinctive albino before 3rd leaf stage but fi-nally turned to normal green after 3rd leaf stage,and the expression of this phenotype was conditionally controlled by develop-ment and temperature.Examining ultrastructure and measuring chlorophyll content indicated that hfa-1 showed albino due to abnormal development of chloroplast,which resulted in inhibiting chlorophyll synthesis and decreasing photosynthetic efficiency.Moreover,the increased tiller number of hfa-1 was ascribed to initiate more higher-order tiller buds.Further endogenous hor-mones analysis demonstrated that the enhanced tillering capacity of hfa-1 might not result from inhibiting the synthesis of IAA.In addition,the dwarfism of hfa-1 was caused by shortening internodes and was independent of biosynthesis and signal transduction of GA.Genetic analysis indicated that the phenotype of green-revertible albino,high-tillering dwarf in hfa-1 was controlled by a recessive nucleic gene,namely hw-1（t）.Using a large F2 mapping population derived from a cross between hfa-1 and an japonica rice variety,02428,hw-1（t） was fine mapped into a 46.9 kb of physical distance between two InDel markers,HW27 and HW7 on chromosome 4,where 13 open reading frames were predicted.In the mapping interval LOC_Os04g57320 encoded a IMMUTANTS protein,which was the most properly candidate gene of hw-1（t）.

关 键 词：水稻 白化转绿 多分蘖矮秆 hw-1(t) 精细定位 

分 类 号：S511[农业科学—作物学]