Studies on Genetic Transformation of NPR1 Gene into Maize by Microprojectile Bombardment  

基因枪介导的玉米NPR1基因的遗传转化(英文)

作　　者：秦新民[1] 李惠敏[1] 曾振华[1] 覃屏生[1] 高成伟[1] 

机构地区：[1]广西师范大学生命科学学院,广西桂林541004

出　　处：《Agricultural Science & Technology》2012年第1期40-43,78,共5页农业科学与技术（英文版）

基　　金：Supported by Natural Science Foundation of Guangxi(0991096)~~

摘　　要：[Objective] This study aimed to explore the conditions of transformation of maize by microprojectile bombardment. [Method] Immature embryo-derived callus of maize inbred line 7239 was used as explants to study the effects of shoot distance, helium pressure, vacuum and bombardment frequency on the transformation efficien- cy in the particle bombardment system of maize. [Result] Considering the transfor- mation efficiency, particle bombardment with 100 μg/P of golden particles, at a shoot distance of 9 cm from the target cells, under helium pressure of 1 350 psi and vac- uum 25 inHg, and bombarding twice could achieve relatively ideal results. After se- lection on media supplemented with different concentration of hygromycin, some re- generated plants were obtained. The results of PCR and Southern blotting analysis demonstrated that the NPR1 gene had been integrated into the genome of trans- genic maize plants, with an average transformation efficiency of 1.76%. [Conclusion] The study laid the foundation for the cultivation and breeding of excellent resistant varieties of maize.[目的]探索出玉米基因枪转化的条件。[方法]以玉米自交系7239的幼胚胚性愈伤组织为受体材料,研究了轰击距离、气体压力、真空度和轰击次数对转化率的影响。[结果]转化时以金粉用量100μg/枪、发射点与靶细胞距离9cm、气体压力1350psi、真空度25In·Hg、轰击次数2次的组合最佳。经潮霉素(Hygromycin)筛选,获得了再生植株,PCR分析及Southern杂交结果表明NPR1基因已整合到玉米基因组中,平均转化率为1.76%。[结论]该研究为玉米优良抗病品种的培育奠定了基础。

关 键 词：MAIZE CALLUS NPR1 gene Particle bombardment Genetic transformation 

分 类 号：S513[农业科学—作物学]