Gd^(3+)与RGD共修饰量子点用于胰腺癌细胞的荧光及MR双模态成像  

Gd^(3+) and RGD Functionalized Quantum Dots for Fluorescent and MR Dual-modality Imaging of Pancreatic Cancer Cells

作　　者：刘峰君[1] 张兵波[2] 宋歌[1] 程英升[1] 

机构地区：[1]同济大学附属第十人民医院影像临床医学中心,上海200072 [2]同济大学先进材料与纳米生物医学研究院,上海200092

出　　处：《高等学校化学学报》2012年第2期378-382,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:51003078);上海市科委基础研究重点项目(批准号:10JC1412900);同济大学青年优秀人才培养计划项目(批准号:2009KJ072);苏州大学现代丝绸国家工程实验室基金资助

摘　　要：结合磁共振成像(MRI)和荧光成像技术,以钆离子(Gd3+)、量子点及精氨酸(R)-甘氨酸(G)-天冬氨酸(D)(RGD)多肽等为功能单元,采用纳米载体组装技术构建了MRI弛豫率/荧光效率高和靶向性强的Gd3+与RGD共修饰的量子点双模态纳米探针(QDs@Gd3+-RGD),并将其用于胰腺癌细胞的双模态成像.实验结果表明,QDs@Gd3+-RGD双模态纳米探针具有较高的弛豫率,且能对胰腺癌patu8988细胞进行荧光和T1-weighted MR成像.Though early diagnosis of pancreatic cancer is difficult,it brings challenges and opportunities to the molecular imaging.The existing diagnostic imaging technologies are limited as a result of single functiona-lity,making it difficult for accurate diagnosis.In this work,magnetic resonance image（MRI） and fluorescence imaging were combined by several key contrast agents including gadolinium ion,quantum dots（QDs） and arginine（R）-glycine（G）-D-aspartic acid（D）（RGD） for high relaxation rate,strong fluorescent emission and good targeting.The integrated dual-modality QDs@Gd3＋-RGD nanoprobes were used for dual-modality imaging of pancreatic cancer cells.TEM and dynamic light scattering（DLS） results indicate complete dispersion of QDs@Gd3＋-RGD nanoprobes in water.In vitro relaxivity measurement shows high relaxation rate of QDs@Gd3＋-RGD nanoprobes.Their r1 and r2 are 7.6 and 9.5 L·mmol-1·s-1,respectively.Fluorescent and MR dual-modality imaging suggest the prepared QDs@Gd3＋-RGD nanoprobes can sever as both fluorescent and MR imaging of patu8988 pancreatic cancer cells.

关 键 词：双模态成像 量子点 RGD 胰腺癌 

分 类 号：O648[理学—物理化学] Q503[理学—化学]