明胶/聚乳酸复合纤维膜的制备、表征及作为角膜细胞载体的评价  

Preparation and Characterization of Gelatin/PLLA Composite Fibrous Scaffold and Evaluation for Corneal Cells Supports

作　　者：颜静[1] 高岩[1] 王清[2] 崔学军[1] 钟双玲[3] 王萍[4] 王洪艳[1] 

机构地区：[1]吉林大学化学学院,长春130012 [2]吉林大学中日联谊医院,长春130033 [3]吉林农业大学资源与环境学院,长春130118 [4]吉林大学第一医院,长春130021

出　　处：《高等学校化学学报》2012年第2期389-394,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:50673032);吉林大学研究生创新基金(批准号:20111035)资助

摘　　要：利用静电纺丝技术制备了明胶与聚乳酸的复合纤维膜,研究了组分配比对复合膜的表面性能、孔隙结构和力学性能的影响,并以复合膜为组织工程支架进行兔角膜上皮细胞的体外培养.采用扫描电子显微镜、免疫荧光染色和噻唑蓝四氮唑溴化物(MTT)比色法综合评价了细胞在支架表面的黏附与增殖能力.结果表明,纺丝溶液的组分对纤维的直径分布和表面亲水性有显著影响,不同组分配比的复合纤维膜均具有高孔隙率的通孔结构;以明胶为基材可维持复合膜的细胞黏附性;与聚乳酸复合可以明显提高复合膜的力学性能.Gelatin and polylactic acid（PLLA） composite membranes were obtained via electrospun.The membranes composition on the surface properties,pore structure and mechanical properties were studied.The membranes were further used as scaffolds for rabbit corneal epithelial cells culture in vitro.The adhesion and proliferation of cells on these scaffolds were evaluated by immunofluorescence staining and thiazolyl bule tetrazolium bromide（MTT） assay.The results show that the fiber diameter distribution and surface hydrophilicity are significantly affected by composition of electrospinning solution.All of these scaffolds have high porosity and inter-connected pores.Gelatin could maintain high cell adhesion while PLLA improved mechanical properties of the composited scaffolds.

关 键 词：明胶 聚乳酸 复合支架 角膜细胞 

分 类 号：O632.5[理学—高分子化学]