基于鱼腥草ISSR扩增条带信息熵的一次投料量研究  

Study on once sampling quantitation based on information entropy of ISSR amplified bands of Houttuynia cordata

作　　者：王海琴[1] 刘文龙[1,2,3] 贺福元[1,3] 陈作红[2] 张喜利[1] 谢相贵[1] 曾姣丽[1] 段晓鹏[1] 

机构地区：[1]湖南中医药大学药学院药学基础教学实验中心,湖南长沙410208 [2]湖南师范大学生命科学院药学基础教学实验中心,湖南长沙410081 [3]中药药性与药效国家中医药管理局重点实验室,湖南长沙410208

出　　处：《中国中药杂志》2012年第3期288-292,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金青年科学基金项目(30901971);国家自然科学基金项目(81073142)

摘　　要：目的:从中药多态性表达的另一种形式——中药基因多态性出发,应用DNA扩增的多态性条带所携带的信息熵探讨鱼腥草一次最小投料量。方法:采用ISSR分子标记对同一GAP产地鱼腥草进行多态性分析,将扩增的条带信息转化成信息熵,运用数学模型测算鱼腥草的最小一次投料量。结果:将筛选出的9条ISSR引物对同一GAP产地46株鱼腥草的DNA样品进行扩增,得到134条总条带,其信息熵H=0.365 6～0.978 6,RSD 14.75%,一次投料量W=11.22 kg(863株)。结论:从基因多态性角度测算出了鱼腥草的另一个"最小投料量",这个量与从指纹图谱角度得到的量存在较大差别,这为探索适宜临床疗效的、能真正指导临床合理用药的"一次投料量"奠定基础,最终实现单味中药剂型改革。Objective： To explore the once sampling quantitation of Houttuynia cordata through its DNA polymorphic bands that carried information entropy, from other form that the expression of traditional Chinese medicine polymorphism, genetic polymorphism, of traditional Chinese medicine. Method： The technique of inter simple sequence repeat （ISSR） was applied to analyze genet- ic polymorphism of H. cordata samples from the same GAP producing area, the DNA genetic bands were transformed its into the infor- mation entropy, and the minimum once sampling quantitation with the mathematical mode was measured. Result：One hundred and thirty-four DNA bands were obtained by using 9 screened ISSR primers to amplify from 46 strains DNA samples of H. cordata from the same GAP, the information entropy was H = 0. 365 6-0. 978 6, and RSD was 14.75%. The once sampling quantitation was W = 11.22 kg （863 strains）. Conclusion： The ＂once minimum sampling quantitation＂ were calculated from the angle of the genetic polymorphism of H. cordata, and a great differences between this volume and the amount from the angle of fingerprint were found.

关 键 词：鱼腥草 中药基因多态性 ISSR 信息熵 一次投料量 

分 类 号：S567.239[农业科学—中草药栽培]