筋脉通对糖尿病大鼠周围神经组织诱导型一氧化氮合酶和硝基酪氨酸表达的影响  

Effect of Jinmaitong capsule on inducible nitric oxide synthase and nitrotyrosine in diabetic rats

作　　者：孙青[1] 梁晓春[1] 王普艳[1] 李伯武[1] 赵丽[1] 黄文智[1] 张彦东[2] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院中医科,北京100730 [2]中国医学科学院基础医学研究所病理中心,北京100005

出　　处：《中国中药杂志》2012年第3期348-352,共5页China Journal of Chinese Materia Medica

基　　金：北京市自然科学基金项目(7082077)

摘　　要：目的:观察中药复方筋脉通对链脲佐菌素诱导的糖尿病大鼠坐骨神经诱导型一氧化氮合酶(inducible nitric ox-ide synthase,iNOS)和硝基酪氨酸(nitro tyrosine,NT)蛋白表达的影响。方法:采用链尿佐菌素一次性腹腔内注射的方法造模,随机分为模型对照组、筋脉通低、中、高剂量组、维生素C组及正常对照组。成模后予灌胃给药,筋脉通低、中、高剂量组分别按成人剂量5,10,20倍给药;维生素C组按成人剂量10倍给药;模型组和对照组予灌服蒸馏水。所有实验大鼠于灌胃16周后处死。采用免疫组化染色法测定坐骨神经iNOS和NT蛋白的表达。结果:糖尿病大鼠坐骨神经iNOS和NT蛋白表达均明显升高(P<0.05,P<0.01);与模型组比较,各治疗组iNOS和NT蛋白表达明显降低(P<0.05,P<0.01),其中筋脉通中剂量组作用明显优于维生素C组(P<0.05,P<0.01)。结论:筋脉通能够下调糖尿病大鼠坐骨神经iNOS和NT的蛋白表达。Objective： To observe the effect of Jinmaitong capsule （JMT）, a compound traditional Chinese medicine, on expressions of inducible nitric oxide synthase （iNOS） and nitro tyrosine （NT） protein in streptozocin-induced diabetic （STZ-DM） rats. Method： Intraperitoneal injection of streptozocin in rats to establish a model. STZ-DM rats were randomly divided into the model control group （ distilled water）, the small-dose JMT group （ JMT at dose of 0. 45g · kg-1· d-1 ）, the medium-dose JMT group （ JMT at doseof0.88 g · kg-1· d-1）, the large-doseJMTgroup （JMTatdoseofl. 75g · kg-1· d-1） and Vitamin C group （ VC at dose of 0. 05 g · kg-1· d-1 ）. Ten normal rats with matching weight and age were selected as the normal control group （distilled water）. After intragastric administration for 16 weeks, the expressions of iNOS and NT in sciatic nerve were detected by the immunohistochemistry method. Result ： The expression levels of iNOS and NT protein in diabetic rats were higher than those in normal rats （ P 〈 0. 05, P 〈 0. 01 ）. Compared with the model group, the levels of iNOS and NT protein in JMT and VC groups were significantly decreased （P 〈 0. 05, P 〈0. 01 ）. Particularly, the medium-dose JMT group showed a better effect than the VC group （P 〈0. 05, P 〈0. 01 ）. Con- clusion： JMT could down-regulate the expressions of iNOS and NT protein of sciatic nerve in diabetic rats.

关 键 词：糖尿病周围神经病变 诱导型一氧化氮合酶 硝基酪氨酸 免疫组化染色 筋脉通 

分 类 号：R285.5[医药卫生—中药学]