番茄溃疡病菌的PCR-DHPLC快速检测  

Detection and identification of Clavibacter michiganensis subsp.michiganensis by PCR-DHPLC

作　　者：吴兴海[1] 陈鹏[1] 左一鸣[1] 张成标[1] 封立平[1] 魏晓棠[1] 

机构地区：[1]山东出入境检验检疫局,山东青岛266002

出　　处：《植物检疫》2012年第1期18-23,共6页Plant Quarantine

基　　金：国家质检总局植物病原检测专项基金(2007IK237)

摘　　要：根据番茄溃疡病菌ITS序列,设计并合成了PCR-DHPLC检测引物,对番茄溃疡病菌及其他病菌共10个标准菌株进行了PCR-DHPLC检测。结果表明,番茄溃疡病菌的PCR-DHPLC检测图谱出现了特异性吸收峰,而其他病菌均未在相同洗脱时间出现吸收峰,说明这种方法具有检测番茄溃疡病菌的特异性。灵敏度实验结果表明,PCR-DHPLC体系与PCR-琼脂糖凝胶电泳体系的检测灵敏度一致。研究表明,PCR-DHPLC方法是一种特异、灵敏、快速的番茄溃疡病菌检测方法。Specific primers were designed basing on the ITS sequence in GenBank.The primers used in this assay were shown to be specific for Clavibacter michiganensis subsp.Michiganensis and did not react with other nine standard bacteria strains.The detection limit of PCR-DHPLC was equal to PCR-agrose gel electrophoresis.PCR-DHPLC is a specific,sensitive,rapid and highly effective method for the detection and identification of Clavibacter michiganensis subsp.Michiganensis.

关 键 词：番茄溃疡病菌 PCR-DHPLC 检测 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]