应用实时荧光PCR检测苹果果实球壳孢腐烂病菌  

Application of real-time PCR for detecting Sphaeropsis pyriputrescens

作　　者：邓丛良[1] 边勇[1] 梁新苗[1] 吕玉峰[1] 

机构地区：[1]北京出入境检验检疫局检验检疫技术中心,北京101312

出　　处：《植物检疫》2012年第1期29-31,共3页Plant Quarantine

基　　金：国家公益性课题(20111035);国家973基金资助(2011CB932803);国家质检总局课题(2011IK157)

摘　　要：根据苹果果实球壳孢腐烂病菌(Sphaeropsis pyriputrescens Xiao&J.D.Rogers)ITS区序列设计特异性引物和TaqMan荧光探针,建立了苹果果实球壳孢腐烂病菌的实时荧光PCR检测方法。该方法能够特异性检测苹果果实球壳孢腐烂病菌,供试的目标菌株检测结果为阳性,而苹果轮纹病菌、苹果炭疽病菌、苹果干腐病菌和苹果褐腐病菌、苹果腐烂病菌等5种对照菌株检测结果为阴性。该方法具有快速、简便、准确的优点,适合于该病害的快速诊断和口岸检验检疫运用。A pair of primers and a TaqMan probe basing on the conserved nucleotide sequence of ITS gene of Sphaeropsis pyriputrescens were designed and synthesized,a novel real-time fluorescent PCR was established to detect Sphaeropsis pyriputrescens.This method was capable of detecting Sphaeropsis pyriputrescens specificly.The detection result of mycelium of Sphaeropsis pyriputrescens by real-time fluorescent PCR showed positive,while that of Botryosphaeria berengeriana f.sp.piricola,Colletotrichum gloeosporioides,Botryosphaeria berengeriana,Sclerotinia fructigena and Nectria galligena showed negative.The reliable,quick and easy-handling detection method is suitable for disease diagnosis and quarantine detection.

关 键 词：苹果果实球壳孢腐烂病菌 实时荧光PCR 检测 

分 类 号：S436.611[农业科学—农业昆虫与害虫防治]