Effects of La^(3+) on osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells  

作　　者：ZHANG Jinchao SUN Jing GU Guangqi HAO Xiaohong LIU Dandan LI Yaping QIN Xinying WANG Shuxiang 张金超;孙静;谷广其;郝晓红;刘丹丹;李亚平;秦新英;王书香(Chemical Biology Key Laboratory of Hebei Province,College of Chemistry and Environmental Science,Hebei University,Baoding 071002,China;Affiliated Hospi-tal of Hebei University,Baoding 071000,China)

机构地区：[1]Chemical Biology Key Laboratory of Hebei Province,College of Chemistry and Environmental Science,Hebei University,Baoding 071002,China [2]Affiliated Hospi-tal of Hebei University,Baoding 071000,China

出　　处：《Journal of Rare Earths》2012年第1期90-93,共4页稀土学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (20971034);Foundation for Key Program of Ministry of Education of China (208018);Returned Scholars of Hebei Province (207041);Natural Science Key Foundation of Hebei Province (B2009000161);Natural Science Foundation of Hebei University

摘　　要：In order to elucidate the action of La3＋ on bone metabolism,effects of La3＋ on the osteogenic and adipogenic differentiation of pri-mary mouse bone marrow stromal cells（BMSCs） were studied by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide（MTT） test,alkaline phosphatase（ALP） activity measurement,mineralized function,oil red O stain and measurement.The results showed that La3＋ pro-moted the proliferation of BMSCs except at 1×10-10 and 1×10-6 mol/L.The effect of La3＋ on the osteogenic differentiation depended on con-centrations at the 7th day,but the osteogenic differentiation was inhibited at any concentration at the 14th day.La3＋ promoted the formation of mineralized matrix nodules except at 1×10-8 and 1×10-5 mol/L.La3＋ inhibited adipogenic differentiation except at 1×10-10 and 1×10-7 mol/L at the 10th day,and inhibited adipogenic differentiation except at 1×10-9 mol/L at the 16th day.These findings suggested that La3＋ might have protective effect on bone at appropriate dose and time.This would be valuable for better understanding the mechanism of the effect of La3＋ on bone metabolism.In order to elucidate the action of La3＋ on bone metabolism,effects of La3＋ on the osteogenic and adipogenic differentiation of pri-mary mouse bone marrow stromal cells（BMSCs） were studied by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide（MTT） test,alkaline phosphatase（ALP） activity measurement,mineralized function,oil red O stain and measurement.The results showed that La3＋ pro-moted the proliferation of BMSCs except at 1×10-10 and 1×10-6 mol/L.The effect of La3＋ on the osteogenic differentiation depended on con-centrations at the 7th day,but the osteogenic differentiation was inhibited at any concentration at the 14th day.La3＋ promoted the formation of mineralized matrix nodules except at 1×10-8 and 1×10-5 mol/L.La3＋ inhibited adipogenic differentiation except at 1×10-10 and 1×10-7 mol/L at the 10th day,and inhibited adipogenic differentiation except at 1×10-9 mol/L at the 16th day.These findings suggested that La3＋ might have protective effect on bone at appropriate dose and time.This would be valuable for better understanding the mechanism of the effect of La3＋ on bone metabolism.

关 键 词：La3＋ bone marrow stromal cells osteogenic differentiation adipogenic differentiation MINERALIZATION rare earths 

分 类 号：Q952[生物学—动物学]