双酚A对大鼠背根神经节神经元钙离子通道电流影响  

Effects of bisphenol A on calcium channel currents in dorsal root ganglion neurons of rats

作　　者：王文娟[1] 王军[1] 钱文溢[1] 朱勤[1] 肖杭[1] 

机构地区：[1]南京医科大学公共卫生学院现代毒理教育部重点实验室,江苏南京210009

出　　处：《中国公共卫生》2012年第2期210-212,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(81072329);江苏省普通高校研究生科研创新计划(CX10B_348Z)

摘　　要：目的探讨双酚A对大鼠背根神经节(DRG)神经元钙离子通道影响。方法 SD大鼠,周龄4～6周,采用酶消化法急性分离大鼠背根神经节神经元,分别采用全细胞膜片钳技术和激光共聚焦技术记录钙电流和KCl激发钙瞬变的变化。结果双酚A(0.1、1、10、100μmol/L)呈浓度依赖性抑制大鼠背根神经节神经元钙电流,对钙通道的半数最大抑制浓度(IC50)为11.41μmol/L;10μmol/L双酚A显著改变Ca2+通道的激活特性,电流激活曲线去极化方向移动(P<0.05),该浓度双酚A同样可以抑制50 mmol/L KCL激发瞬时胞内钙浓度增加。结论双酚A对大鼠背根神经节神经元钙离子通道具有抑制作用。Objective To explore the effects of bisphenol A（BPA） on calcium currents in dorsal root ganglion（DRG） neuron cells of rats.Methods Ca2＋ currents were obtained in DRG neurons by whole-cell patch clamp technique;calcium transients induced by 50 mmol/L KCl were also observed in the neurons with laser scanning confocal microscopy assay,and the effects of bisphenol A on calcium channels were observed.Results BPA at the concentrations of 0.1,1,10,and 100 μmol/L significantly inhibited calcium current in DRG neurons cells,with an IC50 of 11.41 μmol/L.BPA shifted the activation curve of calcium currents toward a depolarizing direction and increased the slope factor of the curve.Additionally,10μmol/L BPA inhibited the increment of intracellular Ca2＋ concentration（i） induced by 50 mmol/L KCl.Conclusion BPA has significant inhibitory effects on calcium current and calcium transient invoked by KCl in dorsal root ganglion neurons.

关 键 词：双酚A(BPA) 钙通道 膜片钳 激光共聚焦 钙瞬变 

分 类 号：R994.1[医药卫生—毒理学]