离子交换树脂对钩藤总生物碱的纯化工艺  

Purification Technology of Total Alkaloids from Uncaria rhynchophylla by Ion-Exchange Resin

作　　者：窦月[1] 周洪雷[1] 齐冬梅[1] 李运伦[2] 陈凯[1] 

机构地区：[1]山东中医药大学,济南250355 [2]山东中医药大学附属医院,济南250011

出　　处：《中国实验方剂学杂志》2012年第3期8-10,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省科技发展计划项目(2008GG2NS02011);国家科技重大专项(2009ZX09301-013);山东省自然科学基金项目(ZR2010HM017);山东省教育厅科技计划项目(J105LL05)

摘　　要：目的:建立阳离子交换树脂纯化钩藤总生物碱的工艺。方法:以钩藤总生物碱为研究指标,考察离子交换树脂分离钩藤总生物碱的工艺条件。结果:选择732型阳离子交换树脂,以1 mL.min-1的流速上样,分别用10 BV水,6 BV 80%乙醇洗脱除杂,最后选用1%氨性80%乙醇洗脱得到钩藤总生物碱,含量达到50%。结论:该方法工艺较简单,总碱含量较高,适合工业化生产。Objective：To establish purification technology of total alkaloids from Uncaria rhynchophylla with cation-exchange resin.Method：Total alkaloids from U.rhynchophylla was selected as indicator,and investigated separation technology conditions of total alkaloids from U.rhynchophylla by ion-exchange resin.Result：Seven hundred and thirty-two cation-exchange resin was chosen,flow rate of sample was 1 mL·min-1 of flow rate,then eluted with 10 BV water and 6 BV 80% ethanol,finally eluted with 1% aqueous ammonia 80% ethanol,the content of total alkaloids from U.rhynchophylla was up to 50%.Conclusion：This technology was simply,the content of total alkali was high,suitable for industrial production.

关 键 词：离子交换树脂 钩藤 总生物碱 纯化 

分 类 号：R283.6[医药卫生—中药学]