红花总黄酮大孔树脂纯化工艺  

Purification Technology of Total Flavonoids from Carthamus tinctorius by Macroporous Resin

作　　者：于国峰[1] 丁嘉信[1] 王超[1] 吕志强[1] 田景振[1] 

机构地区：[1]山东中医药大学药学院,济南250355

出　　处：《中国实验方剂学杂志》2012年第3期39-42,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究大孔树脂精制红花总黄酮的工艺条件。方法:以红花总黄酮吸附率与解析率为指标,考察6种不同型号的大孔树脂对红花总黄酮的吸附与解析能力,确定AB-8为最佳树脂。对AB-8大孔树脂的纯化条件进行优化。结果:最佳工艺条件为以50 g.L-1的质量浓度(药液pH 2.0)、4 BV.h-1的流速上样,药液最佳上样体积为3.3 BV(树脂量),以3 BV30%乙醇2 BV.h-1的速率洗脱。经AB-8处理后的红花总黄酮纯度达54.04%,羟基红花黄色素A纯度达28.76%,分离效果良好。结论:AB-8型大孔吸附树脂能较好地纯化富集红花总黄酮。Objective：To optimize technology conditions and parameters of separation and purification for total phenolic acids from Salvoa miltiorrhiza by macroporous resin.Method：Based on the content and transfer rate of total phenolic acids from S.miltiorrhiza as indexes,determined optimum separation and purification conditions of total phenolic acids from extract.Result： Optimum purification conditions were as follows： HPD100 macroporos resin was used by 20% ethanol as elution agent with ratio of resin to material drug 3∶2,ratio of diameter to height of resin column 1∶3,volume of elution agent was 8 BV,and concentration of sample was 48 g·L-1.In these conditions,purity of total phenolic acid from S.miltiorrhiza could reach above 60%,and transfer rate was about 45%.Conclusion： Macroporous resin method was easy to operate and solvent-saving with good promotion prospects.

关 键 词：红花 总黄酮 大孔树脂 

分 类 号：R283.6[医药卫生—中药学]