丹参总酚酸大孔树脂纯化工艺  

Purification Technology of Total Phenolic Acids from Salvia miltiorrhiza by Macroporous Resin

作　　者：魏冬青[1] 陈绍民[2] 苗建武[1] 丁嘉信[1] 陈智[3] 田景振[1] 

机构地区：[1]山东中医药大学,济南250355 [2]山东省食品药品监督管理局,济南250013 [3]济南市第二人民医院,济南250001

出　　处：《中国实验方剂学杂志》2012年第3期42-44,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究大孔树脂分离纯化丹参总酚酸的工艺条件及参数。方法:以丹参总酚酸的含量和转移率为指标,确定提取液中总酚酸的最佳分离纯化条件。结果:HPD100型大孔吸附树脂纯化丹参酚酸的最佳工艺条件为上样液质量浓度48g.L-1,树脂-药液比3∶2,树脂柱径高比1∶3,20%乙醇洗脱,用量8 BV。丹参总酚酸纯度可达60%以上,转移率约45%。结论:该工艺具有操作简单、节省溶剂等优点,具有较好的推广应用前景。Objective:To optimize technology conditions and parameters of separation and purification for total phenolic acids from Salvoa miltiorrhiza by macroporous resin.Method:Based on the content and transfer rate of total phenolic acids from S.miltiorrhiza as indexes,determined optimum separation and purification conditions of total phenolic acids from extract.Result: Optimum purification conditions were as follows: HPD100 macroporos resin was used by 20% ethanol as elution agent with ratio of resin to material drug 3∶2,ratio of diameter to height of resin column 1∶3,volume of elution agent was 8 BV,and concentration of sample was 48 g·L-1.In these conditions,purity of total phenolic acid from S.miltiorrhiza could reach above 60%,and transfer rate was about 45%.Conclusion: Macroporous resin method was easy to operate and solvent-saving with good promotion prospects.

关 键 词：丹参总酚酸 大孔树脂 分离纯化 

分 类 号：R283.6[医药卫生—中药学]