SPE-HPLC-ELSD法测定复方藏药制剂肝纤消颗粒中黄芪甲苷的含量  

Determination of Astragaloside in Tibetan Medicine Preparation Ganxianxiao Granules by SPE-HPLC-ELSD

作　　者：江宝红[1] 黄一平[2] 鞠建明[2] 刘汉清[1] 蔡雪萍[1] 

机构地区：[1]南京中医药大学,南京210046 [2]江苏省中医药研究院,南京210028

出　　处：《中国实验方剂学杂志》2012年第3期69-71,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省社会发展项目(BS2007089);拉萨市科技局重大科技项目(200414)

摘　　要：目的:建立SPE-HPLC-ELSD法测定复方藏药制剂肝纤消颗粒中黄芪甲苷含量的方法。方法:采用固相萃取系统对肝纤消颗粒进行处理,色谱柱为Alltima C18(4.6 mm×250 mm,5μm),流动相乙腈-水(33∶67),流速1.0 mL.min-1,柱温35℃,ELSD漂移管温度110℃,载气流量2.7 L.min-1,进样量20μL。结果:黄芪甲苷在1.014～20.280μg峰面积积分值自然对数与进样量自然对数有良好的线性关系线,r=0.999 3;平均加样回收率为100.1%,RSD 2.6%。结论:该方法操作简单,准确,重复性好,可用于藏药肝纤消颗粒的质量控制。Objective： To establish an analysis method to determine astragaloside in Tibetan medicine Preparation Ganxianxiao granules by SPE-HPLC-ELSD.Method： Samples were processed by the SPE,and determined on an Alltima C18 column（4.6 mm×250 mm,5 μm）,the mobile phase consisted acetonitrile-water（33∶67） with flower rate at 1.0 mL·min-1 and column temperature at 35 ℃,while drift tube temperature was 110 ℃ of ELSD,by the carrier flow was 2.7 L·min-1,and injection volume was 20 μL.Result： The linear ranges of astragaloside was 1.014-20.280 μg（r=0.999 3）,with the average recovery were of 100.1%（RSD 2.6%）.Conclusion： The method is simple,accurate,reproducible and it can be used for the quality control of Tibetan medicine Preparation Ganxianxiao granules.

关 键 词：藏药 肝纤消颗粒 黄芪甲苷 固相萃取-高效液液色谱-蒸发光检测 

分 类 号：R284.1[医药卫生—中药学]