舒肝颗粒对大鼠肝星状细胞活化增殖的抑制作用  

Effects of Shugan Granule on Proliferation and Activation of Rat Hepatic Stellate Cells in vitro

作　　者：石蕾[1] 尹一然[1] 李昌平[1] 

机构地区：[1]泸州医学院附属医院,四川泸州646000

出　　处：《中国实验方剂学杂志》2012年第3期136-138,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家人事部留学回国人员基金(030079);四川省卫生厅项目(川卫125号)

摘　　要：目的:研究中药舒肝颗粒对体外培养活化大鼠肝星状细胞(HSC)活化增殖的影响,探讨舒肝颗粒抗肝纤维化的作用机制。方法:用空白对照及0.01,0.02,0.04 g.L-1的舒肝颗粒分别处理HSC细胞系HSC-T6 24 h后,MTT比色法检测细胞增殖,光镜下观察HSC形态学变化,免疫组化法测各组α-平滑肌肌动蛋白(α-SMA)表达的变化。结果:舒肝颗粒作用HSC细胞24 h后,0.01,0.02,0.04 g.L-1终浓度肝星状细胞抑制率分别为7.02%,13.33%,20.85%,明显高于空白对照组,且与药物浓度相关。与对照组比较,随着舒肝颗粒药物浓度的增高,HSC的细胞增殖和胞体伸展受到明显抑制;空白对照组,舒肝颗粒0.01,0.02,0.04 g.L-1组α-SMA阳性表达率分别为(62.83±3.42)%,(47.31±2.74)%,(37.79±3.01)%,(25.74±2.83)%,各组与空白对照组相比较差异有统计学意义(P<0.05)。结论:舒肝颗粒抑制肝星状细胞活化、增殖并抑制α-SMA表达,可能是其抗纤维化的作用机制。Objective：To study effect of Shugan granule on proliferation and activation of rat hepatic stellate cells in vitro and investigate the main mechanism of Shugan granule in protection of liver fibrosis.Method： The experiment was divided into the blank control group,Shugan granule 0.01 g·L-1 group,Shugan granule 0.02 g·L-1 group and Shugan granule 0.04 g·L-1 group.HSC was intervented in vitro for 24 h,then cell proliferation were found by MTT,the morphological changes were detected,α-smooth muscle actin（α-SMA） expression was investigated by Immunohistochemical method.Result： After 24 hours of incubation,Shugan granule at concentrations of 0.01,0.02,0.04 g·L-1 reduced the rates of cell growth by 7.02%,13.33%,20.85%,respectively,showing inhibitory effects with concentration-dependent manner.Compared with the control group,cell proliferation and cell body extension was obviously inhibited by Shugan granule,the positive rate of expressed α-SMA in control group was（62.83±3.42）%,in Shugan granule 0.01 g·L-1 group was（47.31±2.74）%,in Shugan granule 0.02 g·L-1 group was（37.79±3.01）%,in Shugan granule 0.04 g·L-1 group was（25.74±2.83）%,among control group and three Shugan granule intervention group there was a significant difference（P0.05）.Conclusion：Shugan granule can resist hepatic fibrosis through inhibiting the activation and prolifition of HSC.

关 键 词：舒肝颗粒 肝星状细胞 肝纤维化 Α-SMA 

分 类 号：R285.5[医药卫生—中药学]