解毒化瘀药调控白血病HL60/adr细胞NF-κB-survivin通路研究  

Regulation of pathway of survivin-NF-κB of leukemia HL60/adr cells by Jiedu Huayu Fang

作　　者：马武开[1] 姚血明[1] 唐芳[1] 周静[1] 李玉莹[1] 黄颖[1] 王莹[1] 

机构地区：[1]贵阳中医学院第二附属医院,贵州550003

出　　处：《北京中医药大学学报》2012年第1期25-28,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.30860353);贵州省高层次人才科研特助项目

摘　　要：目的探讨解毒化瘀药含药血清对白血病HL60/adr细胞NF-κB-survivin信号基因表达的影响,逆转耐药的机制。方法采用半定量逆转录聚合酶链反应(RT-PCR)法检测解毒化瘀药含药血清处理后HL60/adr细胞survivin的表达;Western blot法检测NF-κB/P65、NF-κB/P50、IκBα的表达。结果解毒化瘀药含药血清能够降低HL60/adr耐药细胞NF-κB/P65、NF-κB/P50、IκBα信号,降低survivin基因的表达。结论解毒化瘀药能够降低HL60/adr细胞NF-κB信号通路,影响NF-κB-survivin途径,逆转耐药。Objective To probe into the influences of medicinal serum of Jiedu Huayu Fang on the expressions of survivin gene and NF-κB signal gnen of leukemia HL60/adr cells. Methods The expression of surviving gene of HL60/adr cells treated with medicinal serum of Jiedu Huayu Fang was detected by using semi-quantitative reverse transeription-polymerase chain reaction （RT-PCR）, and expressions of NF-KB/P65, NF-κB/P50 and IκBα were detected by using Western blotting method. Results The medicinal serum of Jiedu Huayu Fang reduced the signals of NF-κB/P65, NF-κB/P50 and IκBα of multidrug resistanee HL60/adr cells, and decreased the expression of survivin gene. Conclusion Jiedu Huayu Fang can reduce NF-κB signal pathway, influence NF-KB-survivin pathway and reverse multidrug resistance of HL60/adr cells.

关 键 词：白血病 多药耐药 HL60/adr细胞 SURVIVIN NF-κB信号 解毒化瘀药 兔 

分 类 号：R259.932[医药卫生—中西医结合]