胃乐散对溃疡大鼠免疫球蛋白、IL-2及IL-4的影响  

Effects of Weilesan on IL-2,IL-4 and immune globulin in gastric ulcers of rats

作　　者：文国容[1] 赵正兰[1] 徐靖宇[1] 刘雪梅[1] 谢睿[1] 江义霞[1] 陈萍[2] 余丽梅[3] 庹必光[1] 

机构地区：[1]遵义医学院附属医院消化内科,贵州遵义563000 [2]遵义医学院附属医院药剂科,贵州遵义563000 [3]遵义医学院贵州省细胞工程重点实验室,贵州遵义563000

出　　处：《华西药学杂志》2012年第1期25-27,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:30860092);贵州省中医药管理局基金资助[黔中医药发(2009)79号]

摘　　要：目的观察胃乐散对实验性溃疡大鼠的免疫球蛋白及白细胞介素2(IL-2)、白细胞介素4(IL-4)的影响,并探讨其作用机制。方法取SD大鼠48只,随机均分为正常组、模型组、胃乐散低、中、高剂量组和雷尼替丁组,采用冰醋酸烧灼法制备大鼠的胃溃疡模型。连续用药14 d后,处死大鼠,取血清,用ELISA法检测血清中IL-2、IL-4,用免疫比浊法测量血清中免疫球蛋白IgG、IgA、IgM水平的变化。结果模型组大鼠的IL-2、IL-4明显低于正常组。与模型组比较,胃乐散能提高大鼠的IL-2、IL-4的水平(P<0.05),也能增加血清中IgG、IgA、IgM的含量(P<0.05)。结论胃乐散治疗胃溃疡的机制之一可能与提高机体的细胞免疫和体液免疫有关。OBJECTIVE To investigate the effects of Weilesan on IL-2,IL-4 and immune globulin in gastric ulcers of rats,and discuss its functional mechanism.METHODS 48 rats were randomly divided into normal group,model group,low dose of Weilesan group,middle dose of Weilesan group,high dose of WeiLesan group and ranitidine group.The gastric ulcer model was induced by the glacial aceticacid.After drug treatment for 14 days,the blood samples were collected to measure the content of IL-2 and IL-4 in serum by ELISA.IgG,IgA,IgM were measured by Immunoturbidimetry.RESULTS Compared to the control,the levels of IL-2 and IL-4 in model group were significantly lower.Welesan elevated the level of IL-2 and IL-4 in serum（P0.05）,also increased the content of IgG,IgA,IgM（P0.05）.CONCLUSION One of the possible mechanism is that Weilesan improves the cellular and humoral immune function to cure the gastric ulcer.

关 键 词：胃乐散 免疫球蛋白 白细胞介素2 白细胞介素4 大鼠 胃溃疡 免疫比浊法 ELISA 

分 类 号：R96[医药卫生—药理学]