沙棘果中总黄酮苷类的提取工艺研究  

Extracting technology of total flavonoid glycosides in fruit of Hippopphae rhamnoides

作　　者：袁媛[1] 张浩[1] 郑苗[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2012年第1期70-72,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:30472153)

摘　　要：目的探讨沙棘果实中总黄酮苷类的最佳提取工艺。方法采用单因素实验和正交实验结合的方法筛选,对乙醇浓度、提取温度、料液比、提取时间进行四因素三水平的正交试验。结果最佳工艺条件为70℃下采用60%的乙醇浸提,固液比1∶15,回流提取(0.5 h×3)。结论首次将水解后的沙棘总黄酮苷元作为沙棘总黄酮提取工艺的评价指标,在分析检测方法上不仅应测定水解前,还应考察测定水解后总黄酮苷元的含量。OBJECTIVE To find out the best method of extracting total flavonoid glycosides in the berries of seabuckthorn.METHODS Ethanol concentration,extraction temperature,ratio of liquid to solid and extraction time were selected by using single factor experiment and orthogonal experiment.RESULTS The optimum extraction conditions were obtained as extraction temperature of 70 ℃,ethanol concentration of 60%,extraction time of 0.5 h for each time,3 times in all,ratio of liquid to solid 1∶15.CONCLUSION Total flavonoid aglycones obtained by hydrolyzing was first used as an index in process optimization of extracting flavonoid glycosides.It＇s necessary to study the total flavonoids in seabuckthorn further.Meanwhile,we should not only determine the content of total flavonoid aglycones before hydrolyzing,but also determine the content of total flavonoid aglycones after hydrolyzing.

关 键 词：沙棘 总黄酮苷 正交试验 提取工艺 

分 类 号：R28[医药卫生—中药学]