HPLC测定菥蓂子中的黑芥子苷  

Determination of sinigrin in Thlaspi arvense by HPLC

作　　者：陈玉[1] 周旻[2] 伍丽萍[2] 陈聪[2] 叶利明[2] 

机构地区：[1]四川大学华西医院,四川成都610041 [2]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2012年第1期94-95,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用HPLC测定菥蓂子中的黑芥子苷。方法色谱柱为DiamonsilTMC18柱(250 mm×4.60 mm,5μm),流动相为乙腈-20 mmol.L-1四丁基硫酸氢铵(pH6)水溶液(20∶80),流速1 mL.min-1,检测波长227 nm,柱温30℃。结果黑芥子苷0.2543～10.17μg与峰面积的线性关系良好(r=0.9999),平均回收率为98.41%,RSD=0.77%,10批药材中黑芥子苷含量的平均值为3.26%。结论所用方法简便、准确、重复性好,可作为菥蓂子药材的质量控制方法。OBJECTIVE To determine the sinigrin in Thlaspi arvense Linn by HPLC.METHODS The sinigrin was separated on a ODS column.The mobile phase consisted of acetonitrile and four butadiene styrene acid hydrogen amines in a ratio of 20∶80,the detected wavelength was 227 nm.RESULTS The linear range for sinigrin was 0.2543-10.17 mg（r=0.9999）,The average recovery was 99.1% with RSD of 0.77%.The average content in ten samples was 3.26%.CONCLUSION This method is simple,accurate and reliable（RSD1.0%）.It can be used for the quality control of the sinigrin in Thlaspi arvense Linn.

关 键 词：高效液相色谱 菥蓂子 黑芥子苷 

分 类 号：R917[医药卫生—药物分析学]