酒客乐对酒精性胃黏膜损伤的疗效及其机制的探讨  

Compound formula Jiukele's efficacy and its mechanisms in prevention and treatment of acute alcoholic gastric mucosa lesion in rats

作　　者：甘爱萍[1] 胡晓英 陈宏慈[1] 

机构地区：[1]湖北省中医院综合科,湖北武汉430061 [2]北京民航医院高干病房

出　　处：《中国中西医结合消化杂志》2012年第1期18-22,共5页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：湖北省教育委员会重点科研项目(No:96A029)

摘　　要：[目的]探讨中药复方酒客乐对酒精性胃黏膜损伤的防治作用及其机制。[方法]观察各组大鼠实验后胃黏膜的形态学变化,评定胃黏膜损伤指数,用硫代巴比妥酸测定胃黏膜组织丙二醛(MDA)含量,计算胃黏膜凝胶指数,用免疫组化法医学图像分析系统半定量测定胃黏膜组织EGF/EGF-R表达水平。[结果]预防组及治疗组中酒客乐大、小剂量亚组胃黏膜损伤指数和MDA含量均明显低于模型对照亚组(P<0.01),胃黏液凝胶指数及胃黏膜EGF、EGF-R表达水平均显著高于模型对照组(P<0.01)。[结论]酒客乐对急性酒精性胃黏膜损伤有防治作用,其作用可能与其增强黏膜防御损伤能力有关。[Objective]To investigate the efficacy of Chinese compound formula Jiukele in the prevention and treatment of acute alcoholic gastric mucosa lesion in the rats and its mechanisms. [Methods]The model was established by perfusing ethonal to the stomach of the rats. Seventy male Wistar rats were divided randomly into 2 groups： treatment group and prophylactic group, and each group subdivided into control group, model group,Jiukele high and low dose group. Con trol group was shared in the treatment group and prophylactic group,altogether 7 groups,ten in each. Gastric mucosal lesion index （LI）, malonaldehyde （MDA） contents and mucus layers thickness were observed. Epidemital growth factor （EGF） and epidemital growth factor receptor （EGF- R）in the cells of gastric mucosa were detected with immunohistochemical method. [Results] In both treatment group and prophylactic group, gastric mucosal LI and MDA contents in Jiukele high and low dose groups were significantly lower than those in the model group（P〈0.01） ;the gastric mucus layers thickness and EGF/EGF-R were significantly higher than those in the model group（P〈0. 01,respectively）. [Conclusion]Jiukele has effects in the prevention and treatment of acute alcoholic gastric mucosa lesion in rats. The mechanism may contribute to its reinforcement of gastric mucosa defensive function.

关 键 词：胃黏膜损伤 酒精性 酒客乐 大鼠 

分 类 号：R322.4[医药卫生—人体解剖和组织胚胎学]