慢性胃炎脾虚湿热证患者的舌苔蛋白质组学初探  

Exploration on Proteomics of Tongue Coating in Patients with Chronic Gastritis of Spleen-asthenia and Damp-heat Syndrome

作　　者：刘晓谷[1] 蔡淦[2] 何磊[3] 程亚伟[4] 王文静[5] 王忆勤[6] 

机构地区：[1]同济大学附属第十人民医院中医科,上海200072 [2]上海中医药大学附属曙光医院,上海200012 [3]河南中医学院基础医学院,河南郑州450008 [4]海南省中医院消化内科,海南海口570203 [5]上海市疾病预防控制中心公共卫生分子生物学研究室,上海200366 [6]上海中医药大学基础医学院,上海201203

出　　处：《上海中医药大学学报》2012年第1期31-35,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市教委科研创新项目(08ZZ63)

摘　　要：目的:比较慢性胃炎脾虚湿热证患者与正常志愿者舌苔蛋白质谱的差异,筛选慢性胃炎脾虚湿热证的特征性舌苔蛋白标志物,并建立特征性舌苔的诊断模型。方法:收集30例慢性胃炎脾虚湿热证患者(实验组)和30例正常志愿者(正常组)的舌苔,经表面增强激光解析离子化飞行时间质谱(SELDI-TOF-MS)检验并筛选舌苔蛋白标志物,经生物信息学分析建立预测模型。结果:在质荷比1 000～15 000范围内检测到189个差异蛋白峰。与正常组相比,实验组的差异蛋白质谱峰表达值较高(P<0.05);通过聚类分析,两组蛋白质谱峰值显示的聚类性质明显不同;利用主成分分析,两组舌苔样本的分布有明显差异,可有效分离;构建的慢性胃炎脾虚湿热证特征性舌苔诊断模型预测正确率为91.67%,灵敏度为100.00%,特异性为83.33%。结论:本研究所构建的特征性舌苔诊断模型能对慢性胃炎脾虚湿热证做出较为准确的判断,可为慢性胃炎脾虚湿热证的临床诊断提供一定的客观依据。Objective： To compare the differences of protein spectrum of tongue coating between patients with chronic gastritis of spleen-asthenia and damp-heat syndrome and healthy volunteers,filter the characteristic protein markers of tongue coating in patients with chronic gastritis of spleen-asthenia and damp-heat syndrome,and establish the characteristic diagnosis model of tongue coating.Methods： The tongue coating of thirty patients with chronic gastritis of spleen-asthenia and damp-heat syndrome（experimental group） and thirty healthy volunteers（normal group） was collected,the protein markers of tongue coating were filtered by surface enhanced laser desorption ionization time of flight mass spectrometry（SELDI-TOF-MS）,and the prediction model was established by bioinformatics analysis.Results： There were 189 different protein peaks in the range of 1 000 to 15 000 of mass-to-charge ratio,and the expressions of different protein peaks in experimental group were higher than that in normal group（P〈0.05）;the clustering natures of protein peaks were obviously different between two groups through clustering analysis;the distribution of tongue coating samples in two groups had significant differences by principal component analysis and could be separated with each other;the predictive correct rate,sensitivity and specificity of the characteristic diagnosis model of tongue coating established in this research were 91.67%,100.00% and 83.33% respectively.Conclusion： The characteristic diagnosis model of tongue coating established in this research can make accurate judgements on spleen-asthenia and damp-heat syndrome of chronic gastritis,and provide some objective basis for clinical diagnosis.

关 键 词：慢性胃炎 脾虚湿热证 舌苔 SELDI-TOF-MS 决策树方法 

分 类 号：R259[医药卫生—中西医结合]