化学发光法快速高通量检测双嘧达莫  

Detection of dipxridamole by peroxyoxalate-based high-throughput chemiluminescence

作　　者：李云云[1] 章竹君[1] 李冉[1] 张建锋[1] 

机构地区：[1]陕西师范大学化学与材料科学学院,陕西省生命分析化学重点实验室,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2012年第1期46-50,共5页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(30470886);中央高校基本科研业务费专项资金项目(GK20091004)

摘　　要：根据过氧化草酸酯与双氧水作用产生的高能中间体能激发荧光物质发光的原理,建立了利用过氧化草酸酯(TCPO)-H2O2-咪唑化学发光体系测定双嘧达莫的新方法.研究了溶剂、酸度、增敏剂及化学发光试剂浓度对化学发光信号的影响.在最佳试验条件下,双嘧达莫浓度在3.3×10-9～1.7×10-6 g/mL范围内其相对化学发光强度与浓度呈良好的线性关系,检出限为1.1×10-9 g/mL.该方法操作简便快速、灵敏度高、试剂用量少,可用于实际样品血清及尿液的检测.Based on the fact that the bis-（2,4,6-trichlorophenyl） oxalate（TCPO）and hydrogen peroxide（H2O2）can produce a high energy intermediate to transfer its energy to fluorescent compound resulting in luminesce,a highly sensitive,simple,rapid,less consuming reagent method for the determination of dipyridamole was constructed.Optimization of the analysis conditions,including solvent,acidity,catalyst,and the concentration of CL reagents,have been investigated.This method exhibited high performance with a linear correlation between luminesce intensity and amount of dipyridamole in the range 3.3×10-9～1.7×10-6 g/mL and the detection limit of 1.1×10-9 g/mL（S/N=3）.The proposed method has been used for successful analysis of the amount of dipyridamole in serum and urine samples.

关 键 词：化学发光分析 双(2 4 6-三氯苯基)草酸酯(TCPO) 双嘧达莫 

分 类 号：O657.3[理学—分析化学]