Prednisolone succinate-glucosamine conjugate:Synthesis,characterization and in vitro cellular uptake by kidney cell lines  

作　　者：Yan Lin Xun Sun Tao Gong Zhi Rong Zhang 

机构地区：[1]Key Laboratory of Drug Targeting of Ministry of Education West China School of Pharmacy,Sichuan Universit),Chengdu 61004l,China

出　　处：《Chinese Chemical Letters》2012年第1期25-28,共4页中国化学快报（英文版）

基　　金：supported by the National Science & Technology Major Project of China(No.2009ZX09310- 002)

摘　　要：Prednisolone succinate-glucosamine（PSG） conjugate,a prodrug for prednisolone,was synthesized and confirmed by NMR and MS spectrum.The stabilities of the prodrug in PBS（pH 2.50,5.00,7.20,and 7.89） were studied.Cytotoxicity and uptake assay of the prodrug were perfomed on HK-2 and MDCK cell lines.The results showed that compared with prednisolone,the PSG not only did not increase the cytotoxicity but also improved the uptake to 2.2 times of prednisolone by the cells.Thus,it indicated that glucosamine might be a potential carrier for kidney-targeting delivery of prednisolone.Prednisolone succinate-glucosamine（PSG） conjugate,a prodrug for prednisolone,was synthesized and confirmed by NMR and MS spectrum.The stabilities of the prodrug in PBS（pH 2.50,5.00,7.20,and 7.89） were studied.Cytotoxicity and uptake assay of the prodrug were perfomed on HK-2 and MDCK cell lines.The results showed that compared with prednisolone,the PSG not only did not increase the cytotoxicity but also improved the uptake to 2.2 times of prednisolone by the cells.Thus,it indicated that glucosamine might be a potential carrier for kidney-targeting delivery of prednisolone.

关 键 词：Glucosamine Prednisolone Prodrug Cytotoxicity Uptake 

分 类 号：TQ464.1[化学工程—制药化工] TQ460.4