气相色谱技术在革兰氏阳性无芽孢厌氧杆菌鉴定中的应用  

Application of gas chromatogrophy in identifying gram-positive spore-free anaerobic bacillus

作　　者：修淑丽[1] 熊德鑫 梁明 钟方虎[2] 谭立[2] 郑伟[2] 

机构地区：[1]解放军304医院创伤中心研究室,100037 [2]空军航空医学研究所

出　　处：《现代仪器》2000年第1期19-20,共2页Modern Instruments

摘　　要：使用气相色谱技术分析革兰氏阳性无芽孢厌氧杆菌的代谢产物,发现此技术在这类菌的菌属确定上有重要意义。一般来说,双歧杆菌其主要代谢产物是乙酸和乳酸,大致上比例为1.5～3:1,而乳酸杆菌属菌种其主要代谢产物是乳酸,而乙酸产量仅为乳酸的1/5～1/10左右。再通过生化性状鉴定,可进一步确定它们的菌种名称。We used gas chromatography examining metabolite of anaerobic bacteria and found gas chrompaiography is of great importance in identification gram positive non-spore anaerobic bacillus genus. Usually,the main metabolite of bificlobacieria is acetic acid and lactic acid approximately in proportion of 3 : 1,whereas lactobacillus' main metabolite is lactic acid,acetic acid is about l/5~1/10 of lactic acid's amount. According to the gas chromatography results and biochemistry analysis,bacteria species can be determined.

关 键 词：厌氧菌 气相色谱 孔酸 乙酸 双歧杆菌 乳酸杆菌 

分 类 号：R372[医药卫生—病原生物学]