检测HBVDNA/Ig-双特异性循环免疫复合物的免疫捕捉法PCR  

Immuno capture PCR for HBVDNA/Ig-two-component-determined circulating immune complexes

作　　者：周裕林[1] 彭宣宪[1] 柳珑[1] 肖军生 岑岭 杨天赐[1] 

机构地区：[1]厦门大学生物系厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室,厦门361005

出　　处：《中国免疫学杂志》2000年第2期71-72,75,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金!3 96 6 0 0 75

摘　　要：目的:建立一种免疫捕捉法PCR技术,研究血清免疫复合物中不同Ig类型抗体结合HBV的情况,并初步探讨其意义。方法:以羊抗人μ、γ、α链的IgG为捕捉抗体,再利用PCR扩增捕捉物中的HBVDNA。结果:成功地建立了检测HBVDNA/IgM、IgG和IgATCIC的免疫捕捉法PCR技术。该技术特异性强、敏感性高、重复性好,可以显著提高HBVDNA的检出率。同时发现,结合HBV的抗体的Ig类型组合有明显差异,三者均阳性最高。结论:说明乙肝患者血清免疫复合物中有较多的病毒颗粒。研究HBVDNA/IgTCIC对乙肝发病机理的深入阐明可能具有重要意义。Objective: To establish an immuno capture PCR for the study of classes of immunoglobulins complexed with HBV and of their significance.Methods:Using goat IgG to human μ、γ、α chain as capture antibodies and prime specific PCR as a detecting method.Results:An immuno capture PCR had been established to detect HBVDNA/Ig TCIC (HBVDNA/IgM TCTC,HBVDNA/IgG TCIC, HBVDNA/IgA TCIC). This method was specific,sensitive and reproducible,by which more patients with positive HBVDNA could be found. It was also characted that there were significantly different positive rates among the types of combinations of IgM,IgG and IgA, in which the group with the three of them was hightest.Conclusion:Conclusion:These findings suggested that more HBV may be hide within circulating immune complexes and the study of HBV DNA /Ig TCIC may play an important role in more understanding of pathogenesis in patients with hepatitis B.

关 键 词：免疫捕捉法PCR HBVDNA 乙型肝炎 TCIC 

分 类 号：R512.620.3[医药卫生—内科学] R392－33[医药卫生—临床医学]