灵芪胶囊对人肺腺癌A549细胞株凋亡基因Bcl-2表达的影响  

The Effect of Lingqi Capsules on the Bcl-2 Expression of Apoptosis Gene of A549 Cell Lines

作　　者：李环[1] 王怀刚[1] 赵鑫[1] 苏云明[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药信息》2012年第1期105-107,共3页Information on Traditional Chinese Medicine

基　　金：黑龙江省攻关课题(GC05C31403)

摘　　要：目的:研究灵芪胶囊含药血清对A549细胞(人肺腺癌细胞)凋亡诱导作用及凋亡相关基因(Bcl-2)表达的影响。方法:采用RT-PCR法检测灵芪胶囊对A549细胞相关凋亡基因(Bcl-2)表达的影响。结果:灵芪胶囊含药血清对A549细胞具有凋亡诱导的作用,可下调Bcl-2基因表达,从而诱导细胞凋亡和细胞的分化。结论:灵芪胶囊能够明显抑制A549细胞的增殖,与通过影响凋亡相关的基因表达有直接的关系。Objective ：To observe the apoptotic induction and the effect of expression on the Bcl -2 gene by Lingqi capsules. Methods ： RT - PCR was used to measure the expression on Bcl - 2 gene in herb serum contai- ning LQC . Results：The herb serum containing LQC could induce and cure A549 cancer and differentiation by reducing the expression of Bcl -2. Conclusions：The herb serum of Lingqi capsules can obviously inhibit the grouth of A549 growth by influencing on the expression of apoptotic related genes.

关 键 词：灵芪胶囊 肺癌 A549 BCL-2 

分 类 号：R285.5[医药卫生—中药学]