棉花内生拮抗菌Paenibacillus xylanilyticus YUPP-1抗黄萎病研究  

Biological Control of Cotton Verticillium Wilt by Endophytic Paenibacillus xylanilyticus YUPP-1

作　　者：周燚[1] 杨廷宪[1] 王斌先[1] 孙正祥[1] 

机构地区：[1]长江大学农学院,湖北荆州434025

出　　处：《棉花学报》2012年第1期71-77,共7页Cotton Science

基　　金：农业部"公益性行业(农业)科研专项-棉花黄萎病的综合防治"(nyzx07-052);农业微生物国家重点试验开放课题"解木聚糖类芽孢杆菌中环糊精糖基转移酶基因的克隆与表达"(AML200806);湖北省教育厅重大项目"抗棉花枯黄萎病生防菌剂‘克萎菌’的研发"(Z20091201);国家大学生创新性实验计划"抗棉花枯黄萎病生防菌的筛选"(091048922)

摘　　要：在棉花现蕾期筛选到一个高抗黄萎病的内生菌菌株,根据16S rDNA序列同源性,将其命名为Paenibacillus xylanilyticus YUPP-1(解木聚糖类芽孢杆菌YUPP-1)。当把该菌标记后,每株棉花施用该菌株达到1×106 cfu时,该菌能顺利定殖于棉花体内。室内盆栽抗病实验表明:当生防菌灌根处理的菌量为每株1×106,1×107,1×108 cfu,其在结铃期的发病株率仅为8%,5%和2%,而此时对照的发病株率高达96%。2008-2009年的小区防治结果表明:当生防菌灌根处理的菌量为每株1×108 cfu时,2008年处理区的棉花黄萎病发病株率和病指为14.8%和8.5,对照分别为53.3%和26.5,2009年处理区的棉花黄萎病发病株率和病指为9.4%和6.5,对照为37.8%和18.8。这些结果表明,解木聚糖类芽孢杆菌YUPP-1对防治棉花黄萎病具有巨大的应用潜力。To control cotton wilt caused by Verticillium dahliae Kleb,we collected endophytic antagonistic microorganisms during different growth stages and assayed the combined effects.One strain with strong activity against V.dahliae was isolated from cotton seedlings.This strain was preliminarily identified as Paenibacillus xylanilyticus YUPP-1 by 16S rDNA sequence analysis.This strain was able to successfully colonize cotton roots when it was at concentrations of more than 1×10^6 cfu per cotton seedling.A pot experiment of the effect of Paenibacillus xylanilyticus YUPP-1 on cotton Verticillium wilt showed the following： in comparison with a control with an infection rate of 96%,only 8%,5%,and 2% infection rate was observed for the treatments with strain YUPP-1,1×10^6,1×10^7,and 1×10^8 cfu per cotton seedling,respectively.Field tests indicated that control of this disease could be achieved when strain YUPP-1 reached 1×108 cfu per cotton seedling.We have thus demonstrated that P.xylanilyticus YUPP-1 is an effective endophytic biocontrol agent for Verticillium wilt.

关 键 词：棉花 黄萎病菌 解木聚糖类芽孢杆菌 定殖 防效 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]