大鼠跟腱末端病的细胞凋亡的检测  

Research of Apoptoses Test on Achilles Tendon—bone Insertion in Wistar Rats

作　　者：胡亚哲[1] 扈盛[2] 王梅[2] 扈克文[2] 

机构地区：[1]华中师范大学体育系,武汉430079 [2]武汉体育学院,武汉430079

出　　处：《中国中医骨伤科杂志》2000年第1期9-11,共3页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：武汉体育学院科研基金!编号:99015

摘　　要：为了深入研究末端病的发病机制，从而探讨其运动训练中的治疗方法。选取了１３只Ｗｉｓｔａｒ大鼠，用电刺激法进行造模后，进行细胞凋亡检测。发现：末端区大部分结构的细胞凋亡明显增多，特别是腱围组织、腱骨关节而软骨、跟腱、纤维软骨区，跟骨和骨髓等部分；跟骨内软骨岛及脂肪垫的凋亡数量明显减少；滑膜囊和钙化软骨区的细胞凋亡无明显变化。总结发现，末端病中发生细胞凋亡的程度与局部血液供应、组织细胞受损伤、组织受牵拉力情况有直接关系。组织细胞承担负荷的能力与所承担的负荷之间的矛盾是本病公认的原因，而承担了超负荷之后，组织细胞的损伤与其恢复之间的矛盾是治疗本病的重要环节。In this study,the electrostimulation was used to cause enthesiopathy in Wistar rats.The disease tissue was subsequently subjected to the detection of cell apoptosis.In this study,two forms of cell injries were observed in enthesiopathic tissue:necrosis ans apoptosis.The number of apoptotic cells increased significantly in most part of Tendon—bone insertion,especially in paratenon,cartilago articalaris,Achilles tendon,unmineralized fibrocartilage,calcaneus and cavites medullaris;whereas there is an obervious decrease of apoptotic cells in cartilage island and fat pad .No obvious change in apoptosis could be seen in bursa synoriale and mineralized fibrocartilage.In sum,the intensity of apoptosis in enthesiopathic tissue is directly correspondent to the local blood supply,the damage of the cells and the stretch loaded on the tissue.The well—accepted cause of enthesiopathy is the imbalance between the ability of tissues to cope with stress and the stress loaded on the tissue.Once the tissue is overloaded with stress,the balance of the cell injury and tissue rehabilitation becomes a key factor of enthesiopathy.

关 键 词：细胞凋亡 运动损伤 跟腱末端病 大鼠 

分 类 号：R686.1[医药卫生—骨科学]