甜瓜黄瓜素基因启动子表达载体的构建及分析  

Functional Analysis of the Cucumisin Gene Promoter in Melon

作　　者：包秋华[1,2] 王磊[1] 牛一丁[1] 哈斯阿古拉[1] 

机构地区：[1]内蒙古大学生命科学学院,呼和浩特010021 [2]内蒙古农业大学乳品生物技术与工程教育部重点实验室,呼和浩特010018

出　　处：《西北植物学报》2011年第11期2153-2157,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家基础科学人才培养基金(J0730648);国家自然科学基金(30660111);国家转基因植物研究与产业化专项(JY04-B-02)

摘　　要：该研究构建了由黄瓜素基因5′端310bp启动子序列驱动β-葡萄糖醛酸酶(GUS)报告基因的植物表达载体pPZP-CGN,通过花粉管通道法将植物表达载体pPZP-CGN导入甜瓜,并采用荧光法定量测定转基因植株中GUS活性。结果显示,gus基因在果实中高表达,而在根、茎、叶等组织中表达活性很低,表明黄瓜素基因上游310bp启动子具有指导外源基因在果实中高效特异表达的特性。In order to study the function of cucumisin promoter in Cucumis melo L.cv.Hetao,a plant expression vector pPZP-CGN was constructed containing the cucumisin 310 bp promoter region,the coding sequence of β-glucuronidase（GUS） as a reporter gene and nos terminator.The melon cultivar Hetao was transformed with pPZP-CGN by pollen tube pathway transformation method.Fluorescence analysis of GUS activity in transgenic melon plants revealed that the promoter was able to direct gus gene expression at high levels in fruits,but almost not in leaves,stems and roots.It was determined that the 310 bp promoter region of cucumisin gene is responsible for high level fruit-specific expression.

关 键 词：甜瓜 黄瓜素 果实特异性 启动子 花粉管通道法 

分 类 号：Q789[生物学—分子生物学]