32个柿主栽品种SSR图谱构建及遗传变异分析  

SSR Fingerprints and Genetic Variations of the 32 Persimmon Major Cultivars

作　　者：艾呈祥[1] 秦志华[1] 陶吉寒[1] 王长君[1] 

机构地区：[1]山东省果树研究所,山东泰安271000

出　　处：《西北植物学报》2011年第11期2185-2191,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：山东省农业良种工程(2010lz010);山东省科技发展计划(2010GNC10906)

摘　　要：以32份柿主栽品种为试验材料,利用SSR标记技术构建其指纹图谱并进行遗传多样性分析。从78对候选引物中筛选出20对多态性高、稳定性好的引物作为核心引物,构建柿主栽品种SSR指纹图谱。结果显示:(1)20对引物在32份材料中共扩增出183条多态性条带,每对引物扩增出3～20条不等,平均每对引物扩增出9.15条,多态性比率为81.3%。各个位点的杂合度在0.410 3～0.914 3之间,平均为0.702 7。(2)8对引物在12个品种上具有特征带型,采用5对引物进行组合鉴定即可将32个柿品种完全区分开。(3)依据SSR带型特征,对每对引物生成的不同带型直接编号,简化柿SSR带型记录方法,并利用每个品种的带型编号,建立其DNA指纹图谱,结果表明,核心引物组合法比特征谱带法更适用于构建中国柿主栽品种DNA指纹图谱。(4)根据系统聚类分析将32个柿主栽品种分为两大类,品种间的亲缘关系与地理来源具有一定的相关性。The aim of this study was to construct a DNA fingerprinting database of 32 persimmon major cultivars,and the genetic diversity was analyzed based on simple sequence repeats（SSR）.Twenty core SSR primer pairs with high polymorphisms and good repeatability were successfully screened from 78 candidates.183 polymorphic bands were amplified from the 32 cultivars by 20 primer pairs,and 9.15 genotypes were detected by each SSR primer pair on an average ranging from 3 to 20.The level of polymorphism was 81.3%,the heterozygosity of each locus ranged from 0.410 3~0.914 3,with an average level of 0.702 7.Twelve cultivars had specific genotypes by 8 primer pairs.32 cultivars could be identified by 5 primer combinations at least.Due to the complex SSR patterns in persimmon,it was difficult to score each band with number 1 and 0.Here,we scored each SSR pattern with an alphanumeric code instead of with pairs of 1 and 0.By arranging all of pattern numbers,we could build the fingerprint code for every persimmon accession.Core primer combination method is better than distinct band method for the construction of DNA fingerprinting of persimmon major cultivars in China.The cultivars used in this study were divided into 2 groups by the cluster software of NTSYS-pc V2.10,which indicated that the genetic relationship of the persimmon cultivars derived from the same district is similar to a certain extent.

关 键 词：柿 主栽品种 SSR 指纹图谱 遗传多样性 

分 类 号：Q346.5[生物学—遗传学]