手掌参组织培养中外植体褐化的影响因素研究  

Study on Influencing Factors on Browning Rate of Explants in Tissue Culture of Gymnadenia conopsea

作　　者：杨爽[1] 方江平[1] 王勇 

机构地区：[1]西藏农牧学院生物技术中心,西藏林芝860000 [2]西藏林芝地区农牧局,西藏林芝860000

出　　处：《贵州农业科学》2012年第1期22-25,共4页Guizhou Agricultural Sciences

摘　　要：为降低手掌参组培过程中外植体的褐化程度,从表面消毒剂和消毒时间、活性炭和VC的使用、暗培养、光照强度、转瓶间隔时间等方面对手掌参外植体的抗褐化方法进行了探索,以期为建立高效稳定的手掌参再生体系提供依据。结果表明:手掌参的芽部用75%酒精表面消毒20s→无菌水冲洗2次→用0.1%升汞消毒处理10min→无菌水冲洗6次→接种于MS+KT 0.5mg/L+ZT 0.5mg/L+NAA 1.0mg/L+AC 1.0g/L+VC2.0mg/L培养基中→暗培养5～10d(3d转瓶1次)→2 000lx光照条件下培养,其生长良好,褐化程度大大减轻,褐化率由93.33%下降为48.28%。The surface disinfactant and sterilized time,active carbon,VC in use,dark treatment time,light intensity and interval time for transferring another bottle were studied to reduce the browning rate of explants in tissue culture of G.conopsea.The results showed that the browning rate could reduce from 93.33% to 48.28% when the explants were sterilized by 0.1% mercury solution for 10 min,the explants were transferred into another bottle every three days and the treated explants were cultured on MS ＋ KT 0.5 mg/L ＋ZT 0.5 mg/L＋NAA 1.0 mg/L＋AC 1.0 g/L＋VC 2.0 mg/L without light at the early time of tissue culture for 5~10 d,light intensity 2 000 lx was the best.

关 键 词：手掌参 外植体 褐化 组织培养 

分 类 号：S567.239[农业科学—中草药栽培]