小麦抗白粉病基因Pm13和Pm21的多重PCR检测  

Multiplex-PCR Detection of Pm21 and Pm13 Genes with Resistance to Powdery Mildew in Wheat

作　　者：陈涛[1] 隋建枢[1] 彭昕[1] 张素勤[1] 徐如宏[1] 

机构地区：[1]贵州大学农学院麦作研究中心,贵州贵阳550025

出　　处：《贵州农业科学》2012年第1期31-35,共5页Guizhou Agricultural Sciences

基　　金：贵州省科技支撑农业攻关计划项目"小麦种质资源创新与利用"[黔科合NY字(2008)3017];贵州省科技支撑农业攻关计划项目"小麦远缘杂交后代种质的评价与利用"[黔科合带帽字(2009)5002];贵州省科技成果重点推广计划项目"贵农18号小麦新品种高产试验;示范及大面积推广应用"[黔科合成字(2009)5031]

摘　　要：为了促进小麦抗白粉病基因聚合体材料在小麦育种及生产中的应用,利用与抗白粉病基因Pm21和Pm13连锁的特异标记,在含有抗白粉病基因Pm21和Pm13的杂交F4代群体中随机挑选114个单株分别进行单一和多重PCR检测。结果表明,多重与单一PCR检测结果一致,在114个供试单株中,有5株具有Pm21,74株具有Pm13的特异标记,其中4个单株同时具有Pm21和Pm13的特异标记。与抗白粉病基因Pm21和Pm13连锁的特异标记可以用于对Pm21和Pm13的多重PCR检测,更加快速地检测出含有抗病基因Pm21和Pm13的累加体,有效应用于辅助选择育种。In order to promote the application of polymers of genes with resistance to powdery mildew in wheat,PCR and Multiplex-PCR of 114 individuals that selected randomly from the offspring of the cross combination between Pm21 and Pm13,with the specific molecular marker linked to powdery mildew resistant gene Pm21 and Pm13,respectively.The result showed that the outcome of PCR and Multiplex-PCR was consistent,five individuals of the 114 conceive Pm21,74 conceive Pm13,and four conceive Pm21 and Pm13 simultaneously.Consequently,the specific molecular marker linked to powdery mildew resistant gene Pm21 and Pm13 in the present study could be used in Multiplex-PCR of Pm21 and Pm13,detecting the polymer of the resistant gene Pm21 and Pm13 and for better application of maker-assisted selection breeding.

关 键 词：小麦白粉病 PM21 Pm13 分子标记 多重PCR 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]