大鼠部分肝切除术后Hedgehog信号分子的表达  

Expression of Hedgehog signaling molecules in rat livers after partial hepatectomy

作　　者：金海[1] 刘慧玲[2] 李学俊[3] 冯智英[4] 王冰[1] 杨建旭[1] 汪根树[1] 吴斌[2] 

机构地区：[1]中山大学附属第三医院肝脏移植中心,中山大学器官移植研究所,广东省器官移植中心,广东广州510630 [2]中山大学附属第三医院消化内科,广东广州510630 [3]中山大学附属第三医院肝病内科,广东广州510630 [4]中山大学附属第三医院病理科,广东广州510630

出　　处：《中国病理生理杂志》2012年第1期90-93,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81170422);广东省科技计划(No.2008A060202007);中山大学重大项目培育计划(No.10ykjc25)

摘　　要：目的:探讨部分肝切除术后Hedgehog(Hh)信号分子的表达及其意义。方法:切除肝脏左叶和中叶以建立SD大鼠部分肝切除术模型。18只雄性SD大鼠随机均分为3组:假手术组(A)、部分肝切除术组1(B)、部分肝切除术组2(C)。A、B组于术后24 h、C组于术后48 h取肝组织,用免疫组化染色法和Western blotting检测肝组织Ki-67、Sonic Hedgehog(Shh)、Indian Hedgehog(Ihh)和Glioblastoma-2(Gli-2)的表达。结果:HE染色显示B、C组可见肝细胞水肿,点状坏死。C组可见处于不同分裂期的细胞。B、C组Ki-67、Shh、Ihh和Gli-2的表达明显高于A组(P<0.01),C组Ki-67、Shh、Ihh及Gli-2的表达高于B组(P<0.01)。结论:部分肝切除术后Hh信号通路被激活,可能参与促进肝脏修复再生。AIM： To detect the expression of Hedgehog signaling molecules in rat livers after partial hepatectomy.METHODS： The model of rat partial hepatectomy was established by resecting the middle and left lobes of the liver.Eighteen male Sprague-Dawley rats were divided into 3 groups： sham operation group（group A）,partial hepatectomy group 1（group B） and partial hepatectomy group 2（group C）.Hepatic tissues were collected 24 h after the operation in group A and group B,and 48 h after the operation in group C.The expression of Ki-67,Sonic Hedgehog（Shh）,Indian Hedgehog（Ihh） and Glioblastoma-2（Gli-2） in the hepatic tissues were detected by immunohistochemical staining and Western blotting.RESULTS： The edema and spotty necrosis in the hepatic tissues were observed in group B and group C by HE staining.The cells of different dividing stages were found in the hepatic tissues of group C.Compared with group A,the expression of Ki-67,Shh,Ihh and Gli-2 in group B（P0.01） and group C（P0.01） was significantly elevated,and the expression levels in group C were higher than those in group B（P0.01）.CONCLUSION： Hedgehog signaling in rat livers may be activated after partial hepatectomy and stimulate liver regeneration.

关 键 词：大鼠 肝切除术 肝再生 HEDGEHOG信号通路 

分 类 号：R332[医药卫生—人体生理学]