Temperature- and concentration-dependence of kainate-induced y oscillation in rat hippocampal slices under submerged condition  

作　　者：Cheng-biao LU Zhi-hua WANG Yan-hong ZHOU Martin VREUGDENHIL 

机构地区：[1]Department of Automation,Institute of Electrical Engineering,Yanshan University,Qinhuangdao 066004,China [2]Department of Computer,Institute of Mathematics and Information Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066004,China [3]Department of Neurophysiology,University of Birmingham,Birmingham,West Midlands,United Kingdom

出　　处：《Acta Pharmacologica Sinica》2012年第2期214-220,共7页中国药理学报（英文版）

摘　　要：Aim： Fast neuronal network oscillation at the y frequency band （y oscillation： 30-80 Hz） has been studied extensively in hippocampal slices under interface recording condition. The aim of this study is to establish a method for recording y oscillation in submerged hip- pocampal slices that allows simultaneously monitoring y oscillation and the oscillation-related intracellular events, such as intracellular Ca2＋ concentration or mitochondrial membrane potentials. Methods： Horizontal hippocampal slices （thickness： 300 pm） of adult rats were prepared and placed in a submerged or an interface chamber. Extracellular field recordings were made in the CA3c pyramidal layer of the slices. Kainate, an AMPA/kainate receptor ago- nist, was applied via perfusion. Data analysis was performed off-line. Results： Addition of kainate （25-1000 nmol/L） induced y oscillation in both the submerged and interface slices. Kainate increased the y power in a concentration-dependent manner, but the duration of steady state oscillation was reduced at higher concentrations of kainate. Long-lasting y oscillation was maintained at the concentrations of 100-300 nmol/L. Under submerged condition, y oscil- lation was temperature-dependent, with the maximum power achieved at 29~Co The induction of y oscillation under submerged condi- tion also required a fast rate of perfusion （5-7 mL/min） and showed a fast dynamic during development and after the washout. Conclusion： The kainite-induced y oscillation recorded in submerged rat hippocampal slices is useful for studying the intracellular events related to neuronal network activities and may represent a model to reveal the mechanisms underlying the normal neuronal synchronizations and diseased conditions.Aim： Fast neuronal network oscillation at the y frequency band （y oscillation： 30-80 Hz） has been studied extensively in hippocampal slices under interface recording condition. The aim of this study is to establish a method for recording y oscillation in submerged hip- pocampal slices that allows simultaneously monitoring y oscillation and the oscillation-related intracellular events, such as intracellular Ca2＋ concentration or mitochondrial membrane potentials. Methods： Horizontal hippocampal slices （thickness： 300 pm） of adult rats were prepared and placed in a submerged or an interface chamber. Extracellular field recordings were made in the CA3c pyramidal layer of the slices. Kainate, an AMPA/kainate receptor ago- nist, was applied via perfusion. Data analysis was performed off-line. Results： Addition of kainate （25-1000 nmol/L） induced y oscillation in both the submerged and interface slices. Kainate increased the y power in a concentration-dependent manner, but the duration of steady state oscillation was reduced at higher concentrations of kainate. Long-lasting y oscillation was maintained at the concentrations of 100-300 nmol/L. Under submerged condition, y oscil- lation was temperature-dependent, with the maximum power achieved at 29~Co The induction of y oscillation under submerged condi- tion also required a fast rate of perfusion （5-7 mL/min） and showed a fast dynamic during development and after the washout. Conclusion： The kainite-induced y oscillation recorded in submerged rat hippocampal slices is useful for studying the intracellular events related to neuronal network activities and may represent a model to reveal the mechanisms underlying the normal neuronal synchronizations and diseased conditions.

关 键 词：y oscillation neuronal network hippocampus kainite submerged slice 

分 类 号：Q954.52[生物学—动物学] TQ321.4[化学工程—合成树脂塑料工业]