人参皂甙Rbl对阿霉素心力衰竭大鼠致心脏纤维化因子表达的影响  

The effects of Ginsenoside-Rbl on myocardial fibrosis related protein and mRNA in rats with adriamycin-induced chronic heart failure

作　　者：孔宏亮[1] 宋丽杰[1] 李占全[1] 袁龙[1] 

机构地区：[1]辽宁省人民医院心脏中心,辽宁沈阳110016

出　　处：《南京医科大学学报（自然科学版）》2012年第1期26-29,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：辽宁省自然科学基金资助项目(201102107)

摘　　要：目的:探讨人参皂甙Rbl(Ginsenosides-Rbl,Gs-Rb1)是否通过心脏抑制心脏纤维化相关蛋白和mRNA介导其改善慢性心力衰竭(chronic heart failure,CHF)大鼠的心肌重构效应。方法:在构建阿霉素CHF大鼠模型成功后,将其随机分为Gs-Rb1组(n=17)给予Gs-Rb1 70 mg/(kg.d)和CHF组(n=15),另外选取同龄健康大鼠作为对照组(n=10)。第4周心脏超声评估全心质量指数(HW/BW)和左室质量指数(LW/BW),应用Western blot和RT-PCR方法检测转化生长因子-β1(transforminggrowth factorβ1,TGF-β1)、结缔组织生长因子(connective tissue growth factor,CTGF)、内皮素-1(endothelin 1,ET-1)等蛋白和mRNA的表达。结果:与对照组相比,Gs-Rb1组全心质量指数(HW/BW,P=0.005)及左室质量指数(LW/BW,P=0.000)均显著改善;与CHF组相比,Gs-Rb1组TGF-β1、CTGF和ET-1蛋白的表达均显著下降(P均<0.05),但均显著高于对照组(P均<0.05);Gs-Rb1组TGF-β1、CTGF和ET-1等mRNA的表达均显著低于CHF组(P均<0.05),但均显著高于对照组(P均<0.05)。结论:在Gs-Rbl改善CHF大鼠心肌重构效应中,Gs-Rbl可通过下调TGF-β1、CTGF、ET-1等蛋白和mRNA等介导其保护作用。Objective：To elucidate whether Ginsenosides-Rbl（Gs-Rb1） improved myocardial remodeling and whether Gs-Rb1 improved myocardial fibrosis related protein/mRNA in rat heart with chronic heart failure（CHF） induced by adriamycin.Methods：CHF models of rats were performed by adriamycin.CHF rats were randomly divided into CHF group（n = 15） and Gs-Rb1 group（n = 17） that received 70 mg/（kg·d） Gs-Rb1.In addition,age-matched health rats were referred as control group（n = 10）.At the 4th week,the heart to body weight（HW/BW） and the left ventricular to body weight（LW/BW） was analyzed by echocardiography.Histology was used to assess myocardial interstitial fibrosis in the heart.The expression of transforming growth factor β1（TGF-β1）,connective tissue growth factor（CTGF） and endothelin 1（ET-1） were detected by Western blot and RT-PCR.Results： Compared to CHF group,Gs-Rb1 significantly improved HW/BW（P = 0.005） and LW/BW（P = 0.000）;The protein expression of TGF-β1,CTGF and ET-1 were more significantly down-regulated in Gs-Rb1 group than in CHF group,which in those both group were significantly higher than those in control group（P 0.05）;The mRNA expression of TGF-β1,CTGF and ET-1 were more significantly down-regulated in Gs-Rb1 group than in CHF group,which in the both group were significantly higher than in control group（P 0.05）.Conclusion：The effects of Gs-Rb1,on improving myocardial remodeling in CHF rats,were fulfilled by increasing protein and mRNA of TGF-β1,CTGF and ET-1.

关 键 词：人参皂甙RBL 心肌重构 转化生长因子-β1 结缔组织生长因子 内皮素-1 

分 类 号：R541.6[医药卫生—心血管疾病]