清络通痹颗粒对类风湿关节炎患者RANKL表达的影响  

Effects of Qingluotongbi Granule on the RANKL expression of RA patients

作　　者：黄艳[1] 俞晓忆[2] 赵娟[1] 严娟[1] 徐德国[1] 周玲玲[1] 沈计荣[3] 陆燕[3] 周学平[1] 

机构地区：[1]南京中医药大学,南京210029 [2]中国药科大学,南京210009 [3]江苏省中医院,南京210029

出　　处：《中华中医药杂志》2012年第2期457-459,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江苏省高等学校大学生实践创新训练计划项目(No.2009);教育部留学回国人员科研启动基金项目(No.2006331);江苏省"333"高层次人才培养工程项目(No.2008)~~

摘　　要：目的:观察清络通痹颗粒(QLT)含药血清对类风湿关节炎(RA)患者核因子κB受体活化子配体(RANKL)表达的影响。方法:原代培养RA患者成纤维样滑膜细胞(FLS),取3-5代FLS分组加药,收集上清及细胞;分离RA患者外周血淋巴细胞(PBL),分组加药,收集上清及细胞。运用ELISA法检测上清中RANKL的表达及运用实时定量RT-PCR检测细胞RANKL的表达。结果:ELISA检测结果表明QLT含药血清作用后,淋巴细胞及滑膜细胞上清中RANKL含量降低。实时定量RT-PCR结果表明QLT含药血清作用后,淋巴细胞及滑膜细胞RANKL表达降低。结论:QLT含药血清可抑制RA患者RANKL的表达,并减少RANKL的分泌,这可能是清络通痹颗粒治疗RA的重要机制之一。Objective： To observe the effects of Qingluotongbi Granule（QLT） on RANKL expression of patients with rheumatoid arthritis.Methods： Fibroblast-like synoviocytes（FLS） from patients with rheumatoid arthritis（RA） were isolated and cultured.The peripheral blood lymphocytes（PBL） from patients with RA were isolated and cultured.Cells and supernatant were collected.Supernatant RANKL level was measured by using ELISA.Cells were collected for analysis of RANKL mRNA by using real time RT-PCR.Results： Supernatant RANKL level of FLS and PBL were decreased significantly after QLT-contained serum treatment.The expression of RANKL mRNA in FLS and PBL were decreased significantly after QLT-contained serum treatment.Conclusion： QLT can inhibit supernatant RANKL level of FLS and PBL,and reduce the expression of RANKL mRNA in FLS and PBL.This may be one of the therapeutic mechanisms of QLT on RA.

关 键 词：清络通痹颗粒 成纤维样滑膜细胞 外周血淋巴细胞 核因子κB受体活化子配体 

分 类 号：R285[医药卫生—中药学]