中药肺岩宁方及其拆方对小鼠Lewis肿瘤生长、肺转移和微血管密度的影响  

Traditional Chinese medicine Feiyanning's effect on tumor growth,lung metastasis and microvessel density of lewis tumor in mice

作　　者：王中奇[1] 邓海滨[1] 张丽曼[1] 王立芳[1] 吴继[1] 徐振晔[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《中华中医药杂志》2012年第2期477-480,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市教委创新项目(No.08YZ61);上海市浦东新区中医领军人才项目(No.PWZ12009-04)~~

摘　　要：目的:观察中药肺岩宁及其拆方对C57BL/6小鼠Lewis肺癌肿瘤生长、肺转移和肿瘤微血管密度的影响,探讨肺岩宁抗肿瘤生长转移的机制。方法:采用C57BL/6小鼠,接种Lewis肺癌细胞株,随机分为对照组、肺岩宁组、益气组、补肾组和抗癌组,每组10只,在接种后第2天开始中药灌胃,灌胃21d后处死,观察肿瘤肺转移情况,取瘤称重后石蜡包埋,免疫组化法测定肿瘤微血管密度(MVD)。结果:肺岩宁的抑瘤率33.4%;补肾组抑瘤率24.2%,抗癌组抑瘤率69.6%。肺岩宁组肺转移数目为(2.91±1.37)个,补肾组肺转移数目为(3.20±1.27)个,均显著低于对照组的(4.76±1.83)个。肺岩宁组MVD为(29.57±2.92)条/200倍视野,抗癌组MVD为(24.23±2.33)条/200倍视野,补肾组MVD为(31.93±4.88)条/200倍视野,明显低于对照组(51.03±3.09)条/200倍视野。结论:补肾中药抑制肿瘤生长和肺转移;抗癌方对肿瘤生长具有显著抑制作用;益气中药可能促进肿瘤生长;抑制血管生成可能是中药肺岩宁方抗肿瘤侵袭转移的机制之一。Objective： To observe the traditional Chinese medicine Feiyanning prescription＇s effect on the tumor growth and lung metastasis of Lewis tumor loaded in C57BL/6 mice,as well as influence on tumor microvessel density（MVD）.Method： 50 mice,inoculated Lewis lung cancer cell strain,were divided randomly into five groups： the control group,Feiyanning（FYN） group,benefiting Qi（BQ） group,tonifying kidney（TK） group,and anti-cancer（AC） group.The mice were perfused stomach by different remedy fluid since the first day after inoculated,and were put to death on the 22nd day.Tumors extracted from mice were embedded in paraffin after weighed.Lung metastases were checked with microscope.The tumor＇s MVD was measured by immunohistochemistry.Result： The restraining tumor rate in FYN group was 33.4%,in TK group 24.2%,in AC group 69.6%.But tumor weight in BQ group exceed to the control group.On the lung metastasis,the number in the FYN group was（2.91±1.37）,in the TK group was（3.20±1.27）.The number in the both group was lower significantly than that in the control group（4.76±1.83）.Tumor MVD in the FYN group was（29.57±2.92）,in the AC group was（24.23±2.33）,in the TK group was（31.93±4.88）.These numbers were smaller markedly than that in the control group（51.03±3.09）.Conclusion： TK prescription can inhibit tumor growth and anti-metastasis;AC prescription inhibits tumor growth significantly;Chinese medicine BQ prescription may contribute to tumor growth.FYN prescription can reduce the tumor MVD,inhibiting angiogenesis may be one of the mechanisms during anti-tumor invasion and anti-metastasis.

关 键 词：肺岩宁方 益气补肾抗癌 LEWIS肺癌 肿瘤转移 微血管密度 

分 类 号：R285.5[医药卫生—中药学]