Array-ELISA法和ECLIA法测定六项肿瘤标志物的比较研究  

Comparison research of Array-ELISA and ECLIA in determination of six tumor markers in serum

作　　者：陈曲波[1] 黄妩姣[1] 黎翠翠[1] 蒋莉[1] 丁海明[1] 肖倩[1] 黄素丹[1] 

机构地区：[1]广东省中医院检验医学部,广东广州510120

出　　处：《检验医学》2012年第1期4-7,共4页Laboratory Medicine

基　　金：广东省科技厅社会发展计划资助项目(2008B060600042)

摘　　要：目的分析和评价6项肿瘤标志物测定试剂盒[微阵列酶联免疫法(Array-ELISA)]在肿瘤标志物检测中的应用价值。方法采用Array-ELISA和电化学发光免疫分析法(ECLIA)检测相同标本的6项临床常见肿瘤标志物:甲胎蛋白(AFP)、癌胚抗原(CEA)、前列腺特异性抗原(PSA)、糖类抗原125(CA125)、糖类抗原199(CA199)和糖类抗原153(CA153)。采用受试者工作特征(ROC)曲线进行分析。结果 2种方法检测AFP、CEA、PSA和CA125的阳性率差异无统计学意义(P>0.05),检测CA199和CA153的阳性率差异有统计学意义(P<0.01)。2种方法对AFP、CEA、PSA、CA125和CA199的检测结果总符合率均>85%,仅CA153的总符合率为61.1%。2种方法对6项肿瘤标志物的检测结果显著相关(P<0.01),相关系数(r)为0.695～0.960。2种方法检测AFP、CEA、PSA、CA125和CA199对相关肿瘤诊断的ROC曲线下面积介于0.62～0.99之间;用Array-ELISA检测CA153对乳腺癌诊断的ROC曲线下面积低于ECLIA。结论 Array-ELISA与ECLIA对6项肿瘤标志物的检测结果均有良好一致性,可以引入Array-ELISA检测6项肿瘤标志物作为常规体检项目和筛查项目。Objective To analyze and evaluate the clinical application of tumor marker detection kit [microarray enzyme-linked immunosorbent assay（Array-ELISA）] in determining 6 tumor markers.Methods Electro-chemiluminescence immunoassay（ECLIA） analyzer was used to detect 6 clinically common tumor markers： alpha fetoprotein（AFP）,carcinoembryonic antigen（CEA）,prostate specific antigen（PSA）,carbohydrate antigen 125（CA125）,carbohydrate antigen 199（CA199） and carbohydrate antigen 153（CA153）.The receiver operating characteristic（ROC）curve was evaluated.Results There were no significant differences in the positive rates of AFP,CEA,PSA and CA125 by the 2 methods（P〉0.05）,while the positive rates of CA199 and CA153 had statistical significance（P〈0.01）.The determination results of AFP,CEA,PSA,CA125 and CA199 by the 2 methods showed high total coincidence rate（85%）,and the total coincidence rate of CA153 was 61.1%.The correlation was significant [P〈0.01,correlation coefficient（r）=0.695-0.960],and areas under ROC curves of AFP,CEA,PSA,CA125 and CA199 were 0.62-0.99.The area under ROC curve of CA153 by Array-ELISA was lower than that of ECLIA in the diagnosis of breast carcinoma.Conclusions Both Array-ELISA and ECLIA show good coincidence in detecting the 6 tumor markers,and Array-ELISA could be introduced for clinical routine examination and screening testing of determining 6 tumor markers.

关 键 词：微阵列酶联免疫法 电化学发光法 方法学比较 肿瘤标志物 

分 类 号：R446.61[医药卫生—诊断学]