微量样品中喜树碱含量的高效液相色谱测定  

Determination of Camptothecin in Milligram Samples by HPLC

作　　者：王轶[1] 吴莹[1] 王洋[1] 阎秀峰[1] 

机构地区：[1]东北林业大学盐碱地生物资源环境研究中心/东北油田盐碱植被恢复与重建教育部重点实验室(东北林业大学),哈尔滨150040

出　　处：《东北林业大学学报》2012年第2期100-102,共3页Journal of Northeast Forestry University

基　　金：高等学校博士学科点专项科研基金(20090062110008)

摘　　要：为了测定微量喜树(Camptotheca acuminata Decne)样品中的喜树碱含量,建立了HPLC—荧光检测测定方法(采用Luna C18色谱柱(250 mm×4.6 mm,5μm),以乙腈∶水(体积比4∶6)为流动相,流速1.0 mL.min-1,荧光检测器激发波长380 nm,发射波长553 nm)。结果表明:喜树碱质量浓度在20～800μg.L-1范围内,与峰面积呈现良好的线性关系,r=0.999 6,平均加样回收率为98.41%,峰面积的相对标准偏差1.88%。这一方法仅需2 mg干质量植物样品即可实现对喜树碱含量的测定,且操作简单、重复性好、结果精确可靠,为微量植物材料中喜树碱含量的测定提供了有效方法。To determine the content of camptothecin（CPT） in milligram samples of Camptotheca acuminata seedlings,a HPLC method with fluorescence detection was established.The analysis was carried out on a Luna C18 column（250 mm×4.6 mm,5 μm） with the mobile phase of acetonitrile/water（4∶6 by volume） at the flow rate of 1.0 mL·min-1.Fluorescence detector was used for the detection of CPT with excitation and emission wavelengths of 380 nm and 553 nm.The calibration curve was linear at the range of 20-800 ng·mL-1 for camptothecin（r=0.999 6）.The average recovery was 98.41%,and the relative standard deviation was 1.88%.The method is simple,reproducible,reliable and sensitive for only 2 mg（dry weight） of the plant material.It is suitable for accurate determination of camptothecin content in milligram samples of C.acuminata seedlings.

关 键 词：喜树 喜树碱 高效液相色谱法 荧光检测 

分 类 号：S718.43[农业科学—林学] Q946.88[生物学—植物学]