温度和盐度对吉富品系尼罗罗非鱼幼鱼鳃Na^+-K^+-ATPase活力的联合效应  

Combined effect of temperature and salinity on the Na^+-K^+-ATPase activity from the gill of GIFT tilapia juveniles(Oreochromis niloticus)

作　　者：王海贞[1] 王辉[1] 强俊[2] 徐跑[2] 李瑞伟 

机构地区：[1]广东海洋大学水产学院,湛江524025 [2]中国水产科学研究院淡水渔业研究中心,无锡214081 [3]茂南三高罗非鱼良种繁殖基地,茂名525024

出　　处：《生态学报》2012年第3期898-906,共9页Acta Ecologica Sinica

基　　金：国家科技支撑计划项目(2008BADB9B01-3);农业部公益性行业科研专项(200903046-02);广东省教育部产学研结合项目(2008B090500088;2010B090500032)

摘　　要：试验采用中心组合设计(central composite face-centered design,CCF)和响应曲面法(response surface methodology,RSM)研究了温度(12—34℃)和盐度(0—26)两因素对体长为(4.36±0.105)cm,体重为(2.45±0.153)g的吉富品系尼罗罗非鱼(GIFT Nile tilapia,Oreochromis niloticus;简称吉富罗非鱼)幼鱼鳃Na+-K+-ATPase活力的联合效应。结果表明:(1)温度和盐度的一次效应和二次效应对Na+-K+-ATPase活力影响极显著(P<0.01),温度和盐度的互作效应不显著(P>0.05);(2)经响应曲面法分析,随着温度和盐度的增大,Na+-K+-ATPase活力呈先减小后增大的趋势;(3)建立了Na+-K+-ATPase活力与温度、盐度间关系的模型方程(R2=0.9829,Pred.R2=0.8550,P<0.01),并可用于预测吉富罗非鱼幼鱼鳃Na+-K+-ATPase的活力;(4)优化结果显示,温度为24.15℃,盐度为11.75时,Na+-K+-ATPase活力最小为0.62μmol无机磷.mg-1蛋白.h-1,满意度函数值高达0.961。Na+-K+-ATPase活力可以作为检测罗非鱼生长性能的指标,其活力较低时,一般反映了鱼体生存环境适宜,生长代谢旺盛,消耗于渗透调节的能量较少。Central composite face-centered design（CCF） and response surface methodology were used in the experiment to examine the combined effect of temperature（12—34℃） and salinity（0—26） on the Na＋-K＋-ATPase activity from the gill of tilapia juveniles（GIFT strain）（mean body length（4.3±0.105）cm and mean body weight（2.45±0.153）g）.The results showed that：（1）The linear and quadratic effects of temperature and salinity on the Na＋-K＋-ATPase activity were highly significant（P0.01）,and the interactive effect between temperature and salinity was nonsignificant（P0.05）;（2）Using the response surface method,the Na＋-K＋-ATPase activity varied with increasing temperature and salinity in a curvilinear way;（3）The model equation of the enzyme activity towards temperature and salinity was established,with R2=0.9829 and Pred.R2=0.855（P0.01）.The model equation could be practically used for forecasting the gill Na＋-K＋-ATPase activity in GIFT Nile tilapia juveniles;（4）Via applying statistical optimization of the response of interest,results showed that the optimal factor combination was 24.15℃/11.75,at which the minimum Na＋-K＋-ATPase activity,0.62μmolPi·mg-1pr·h-1,was reached,with the desirability as high as 0.961.The Na＋-K＋-ATPase activity can be used as an indicator of growth of GIFT Nile tilapia juveniles.In general,It can be said that the environment in which the Na＋-K＋-ATPase activity is weaker is suitable for the tilapia juvenile growth,and in this case the metabolism of tilapia is more efficient,and the energy consumed for osmoregulation is smaller.

关 键 词：吉富罗非鱼 温度 盐度 Na+-K+-ATPase 响应曲面法 

分 类 号：S917.4[农业科学—水产科学]