鸡新城疫、传染性支气管炎、禽流感(H9亚型)三联灭活疫苗对禽流感H9亚型流行株攻毒的保护作用  

Protective efficiency of the inactivated Newcastle disease virus,infectious bronchitis virus and avian influenza virus(H9 subtype)vaccine against epidemic strains of avian influenza virus H9

作　　者：林绮萍[1] 陈瑞爱[1] 黄文科[1] 区德庆[1] 严洁珍 

机构地区：[1]广东大华农动物保健品股份有限公司,广东新兴527400

出　　处：《畜牧与兽医》2012年第1期12-15,共4页Animal Husbandry & Veterinary Medicine

摘　　要：为了监测鸡新城疫、传染性支气管炎、禽流感(H9亚型)三联灭活疫苗(LaSota株+M41株+SS/94株)对H9亚型禽流感病毒流行毒株的免疫保护效果,采用H9亚型禽流感病毒SS/94株及2009—2010年现地分离的3株H9亚型禽流感病毒对已免疫上述三联灭活苗的SPF鸡进行攻毒试验。结果显示,试验鸡以0.3 mL/只的剂量免疫三联灭活苗后21 d,其H9亚型禽流感病毒的HI抗体效价可达8～11log2,此抗体水平可抵抗2×106EID50的H9亚型禽流感病毒SS/94株、BLCN09株、WDZ09株、YT10株的攻击,攻毒保护率均达90%(9/10)以上。可见,以SS/94株作为禽流感疫苗抗原制备的三联灭活苗具有良好的免疫原性,能使免疫鸡抵抗2009—2010年期间现地分离的多株H9亚型禽流感病毒的攻击。To monitor the protective efficiency of the inactivated Newcastle disease virus, infectious bronchitis virus and avian influenza vi- rus H9 subtype （AIV-H9） vaccine （LaSota ＋ M41 ＋ SS/94 ） , SPF chickens were respectively inoculated with strain SS/94 and three epi- demic strains of AIV-H9 isolated during 2009--2010, after being immunized with the inactivated vaccine. The results showed that at 21 days after immunization, the HI antibody titers to AIV-H9 in the experimental chickens varied from 8log2 to 1 llog2. The antibody levels had pro- tective ability against the challenge with 2x 106 EIDs0 of AIV-H9 strains including SS/94, BLCN09, WDZ09 and YT10, and the protection rates were above 90% （9/10）. It suggested that the triple inactivated vaccine with the strain SS/94 used as the AIV antigen could induce protective immunity against challenge with epidemic strains of AIV-H9.

关 键 词：鸡新城疫 传染性支气管炎 禽流感(H9亚型) 三联灭活苗 H9亚型禽流感病毒流行毒株 保护效力 

分 类 号：S852.4[农业科学—基础兽医学]