大鼠自体原位肝移植后脑组织超微结构的改变及氧化应激特点  

Changes of cerebral ultrastructure and oxidative damage in rats after orthotopic liverautotransplantation

作　　者：夏华[1] 蔡珺[1] 池信锦[1] 张瑷兰[1] 

机构地区：[1]中山大学附属第三医院麻醉科,广州510630

出　　处：《中华神经医学杂志》2012年第2期141-144,共4页Chinese Journal of Neuromedicine

基　　金：广东省科技计划资助项目（20098030801125）

摘　　要：目的观察自体原位肝移植后SD大鼠脑组织病理学改变、氧化应激与炎症反应特点。方法雄性SD大鼠24只按随机数字表法分为对照组（C组）、再灌注6h模型组（M1组、及再灌注24h模型组（M2组），每组各8只。对照组在麻醉后只进行开腹和血管分离，不进行肝脏的阻断和灌注；M1、M2组则进行自体原位肝移植手术，并分别在肝脏再灌注后6h或24h时取脑组织．透射电镜观察其病理形态学特点，ELISA法检测白细胞介素．6（IL-6）、白细胞介索．10（IL-10）、肿瘤坏死因子-a（TNF-a）水平，分光光度计方法检测超氧化物歧化酶（SOD）、丙二醛（MDA）、过氧化氢酶（CAT）含量。结果在肝脏再灌注以后，M1、M2组大鼠脑组织超微结构电镜下显示为：神经元变形，胞核变形、表面呈锯齿状，核内异染色质增多，核膜及核周隙模糊不清，核仁网状结构消失．线粒体呈气球样肿胀，嵴减少或消失等，且该损伤性改变以M2组更为显著。肝脏再灌注后大鼠脑组织氧化损伤及炎症反应指标都有明显改变，与C组比较，M2组SOD明显降低，差异有统计学意义（P〈0．05）；MI组CAT明显降低，差异有统计学意义（P〈O．05）；M1组MDA、IL-6、IL-10、TNF-a明显升高，差异均有统计学意义（P〈0．05）。结论SD大鼠经自体原位肝移植后，脑组织结构出现明显的病理学改变，同时伴有显著的氧化损伤及炎症反应。Objective To observe the changes of cerebral ultrastructure, and cerebral oxidative damage and inflammation reaction in rats after orthotopic liver autotransplantation. Methods Twenty-four male SD rats were randomly divided into control group （Group C, n=8）, 6-h-reperfusion group （Group M1, n=8）, and 24-h-reperfusion group （Group M2, n=8）. Rats in the Group C received sham operation after anesthesia, involving laparotomy and vascular separation; rats in the Group M1 and M2 received orthotopic liver autotransplantation, and their brain tissues were obtained 6 and 24 h after reperfusion. The changes of cerebral morphology were observed by transmission electron microscope; the levels of interleukin-6 （IL-6）, IL-10 and ~rnor necrosis factor-a （TNF-a） were measured; the levels of superoxide dismutase （SOD）, malondialdehyde （MDA） and catalase （CAT） were observed. Results As compared with those in Group C, the cerebral ultrastructure of Group M1 and Group M2 showed obvious traumatic changes, including microvascular edema and deformation, neuron edema, nuclear deformation with indentation in the surface, increment of heterochromatin in the nuclear, blurring in the karyolemma and perinuclear space, disappearance of reticulate structure in the nucleolus, mitochondria edema and disappearance of endoplasmic reticulum; and these changes in Group M2 were more obvious than those in Group M2. The changes of oxidative damage and inflammatory responses were obvious： as compared with that in Group C, the SOD level in M2 decreased significantly （P〈0.05）, the CAT level in M1 decreased significantly （P〈0.05）, and the levels ofMDA, IL-6, IL-10 and TNF-a in Group M1 increasedsignificantly （P〈0.05）. Conclusion After orthotopic liver transplantation, the pathology changes of cerebral tissues in these SD rats were significant, with obvious oxidative damage and inflammatory reaction.

关 键 词：肝移植 超微结构 氧化损伤 炎症反应 

分 类 号：R657.3[医药卫生—外科学]