三聚氰胺在中华鳖组织中的代谢动力学研究  

Pharmacokinetics of Melamine in tissues from Pelodiscus sinensis

作　　者：何欣[1,2] 张海琪[2] 施礼科 梁丽军[3] 姚高华[2] 

机构地区：[1]浙江工商大学食品与生物工程学院,浙江杭州310035 [2]浙江省水产质量检测中心,浙江杭州310012 [3]浙江工业大学化学工程与材料学院,浙江杭州310032

出　　处：《中国兽医学报》2012年第2期277-280,共4页Chinese Journal of Veterinary Science

基　　金：浙江省农业标准化重点研究项目(2009-16);浙江省科技厅农业重大项目(2007C02002-2)

摘　　要：应用液相色谱-串联质谱监测技术研究了三聚氰胺药饵多次给药后在中华鳖体内的药物代谢动力学。结果表明,中华鳖血液、肝脏、肌肉中三聚氰胺浓度分别在8、4、2h时达到高峰;最高峰时血液、肝脏、肌肉中药物质量浓度平均分别为16.98、16.79和12.05mg/kg。在血液、肝脏和肌肉组织中三聚氰胺浓度的t1/2(ke)分别为24.11、107.75和36.49h。三聚氰胺在血液、肝脏、肌肉组织中的代谢过程均符合一室模型,相应的动力学方程分别为:C血液=17.08×(e-0.0287t-e0.0558t),C肌肉=11.64×(e-0.0190t-e2.1425t),C肝脏=15.72×(e-0.0064t-e2.5558t)。说明药饵给药后三聚氰胺在中华鳖体内消除较慢。The pharmacokinetics of melamine metabolite in Chinese soft-shelled turtle（Pelodiscus sinensi） were analyzed by using HPLC-MS/MS method after artificial feeding.The turtles were fed with the experimental feed containing melamine of 500 mg/kg,for 7 d.Samples were collected after 0.5,1,2,4,8,12,24,48,72,96,120,168 h,and 10,15,30 d,respectively.Each time six samples that included blood,liver and muscle samples were selected random.The samples were placed in the centrifuge tubes,stored in-18℃ refrigerator with 6 blank samples as control group.Through 15 mL 1% trichloroacetic acid solution and 2 mL acetonitrile extraction,samples were determined by HPLC-MS/MS.The data received were analysed by 3P97 pharmacokinetic data analysis software.The change of the concentration of melamine presents a single-peak phenomenon with a slightly difference in different tissues.The concentration of melamine in the blood changed from 0.5 h of 7.94 mg/kg to 8 h of the peak 16.98 mg/kg,then declined to the residue of 0.098 mg/kg after feeding feeding 30 d;The concentration of melamine in the liver was from the 0.5 h of 12.89 mg/kg to 4 h of the peak 16.79 mg/kg,then declined to the residue of 1.19 mg/kg after 30 d.The concentration of melamine in muscle after feeding of from 0.5 h of 7.77 mg/kg to 2 h of the peak to 12.05 mg/kg,then gradual declines to the residues of 0.179 mg/kg after 30d.Pharmacokinetics equations are Cblood=17.08×（e-0.0287t-e0.0558t）,Cmuscle=11.64×（e-0.0190t-e2.1425t）,Cliver=15.72×（e-0.0064t-e2.5558t）,respectively.Elimination half-life t1/2（ke） of melamine in the Chinese soft-shelled turtle liver,blood and muscle were 107.8,24.11,36.49 h,respectively,indicating that its elimination is more slowly.

关 键 词：中华鳖 液相色谱-串联质谱 三聚氰胺 代谢动力学 

分 类 号：S816.7[农业科学—饲料科学]