细胞周期素家族基因在家蚕4龄幼虫蜕皮过程中的转录特征  

Transcriptional Characteristics of Cyclin Family Genes in Molting Process of the 4th Instar Bombyx mori Larvae

作　　者：孙文娴[1,2] 彭广大[1,2] 王玮玥[1,2] 刘丽华[3] 王燕红[1,2] 沈卫德[1,2,4] 李兵[1,2,4] 

机构地区：[1]现代丝绸国家工程实验室,江苏苏州215123 [2]苏州大学医学部,江苏苏州215123 [3]通化师范学院生物系,吉林通化134000 [4]苏州大学蚕桑研究所,江苏苏州215123

出　　处：《蚕业科学》2012年第1期46-50,共5页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目(No.31072086);苏州市科技项目(No.SYN201009);苏州大学第十三批大学生课外学术基金(No.KY2011079A);江苏高校优势学科建设工程项目

摘　　要：细胞周期蛋白(cyclin)是真核生物细胞有丝分裂的重要调节蛋白,家蚕具有5种类型细胞周期蛋白基因。以4龄将眠蚕、4龄眠蚕和5龄起蚕为材料,采用定量PCR方法分析5种细胞周期蛋白基因在4龄幼虫蜕皮过程的组织表达特征为:5种类型cyclin基因在生殖腺中都高表达,其中cyclinE在眠中表达量最高,其它4种类型cyclin基因在将眠时表达量最高,证实4龄眠期是家蚕生殖腺细胞发生有丝分裂的重要时期;5种类型cyclin基因在马氏管中都有表达,其中cyclinA和cyclinB3在4龄将眠蚕的马氏管中存在表达高峰,推测和马氏管内表皮更新相关;除cyclinL1在眠蚕丝腺的表达量很低外,cyclinA、cyclinB、cyclinB3和cyclinE在眠蚕丝腺中都存在表达高峰,可能和丝腺的内表皮更新相关;cyclinA、cyclinB、cyclinB3在脂肪体中的表达量较高,且在将眠时或眠中存在表达高峰,推测和脂肪体中能量物质的积累相关;5种类型cyclin基因在脑、血液和中肠的表达量都很低,且蜕皮前后的变化规律不明显。研究结果提示:在家蚕4龄幼虫蜕皮过程中,cyclin基因的转录特征不仅和来源于外胚层组织的表皮更新相关,还和生殖腺的发育及脂肪体中能量物质的积累存在相关性。Cyclin is an important regulatory protein in mitosis of eukaryotic cells.Silkworm（Bombyx mori） has 5 types of cyclin genes.We used the 4th instar pre-molting,4th instar molting and 5th instar newly exuviated larvae as materials to analyze the tissue expression profile of 5 cyclin genes during the molting and metamorphotic process of the 4th instar silkworm larvae by quantitative PCR.The results showed that all 5 types of cyclin genes were highly expressed in gonad,and cyclinE had an expression peak at the 4th instar molting stage,while the other 4 types of cyclin genes had expression peaks at the 4th instar pre-molting stage,confirming that the 4th molting stage is an important period in the mitotic process of gonad cells.These 5 types of cyclin genes were all expressed in the Malpighian tubule,while cyclinA and cyclinB3 had an expression peak in the Malpighian tubule of 4th instar pre-molting larvae,which might be related to epidermis replacement in the Malpighian tubule.Except cyclinL1 which was lowly expressed in silk gland of the 4th instar molting larvae,cyclinA,cylcinB,cyclinB3 and cyclinE each had an expression peak in silk gland of the 4th instar molting larvae,which might be associated with epidermis replacement of silk gland.The expression levels of cyclinA,cyclinB and cyclinB3 were high in fat body,and reached peak levels at the 4th instar pre-molting or 4th instar molting stage,suggesting that these genes were related to the accumulation of energy substance in fat body.All 5 types of cyclin genes had relatively low expression levels in brain,blood and midgut,and the variation pattern was not obvious before and after molting.These results indicate that in the molting process of the 4th instar larvae,transcriptional characteristics of cyclin genes are not only related to the replacement of the ectodermic tissue originated epidermis,but also related to the development of gonad and the accumulation of energy substance in fat body.

关 键 词：家蚕 细胞周期素 蜕皮变态 组织 基因转录 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]