DMF运载5-氟尿嘧啶在大鼠体内分布浓度的定量检测  

Determination of fluorouracil in rats intervention with a DMF transfer model

作　　者：苟国敬[1] 鲍凤娟[1] 刘彦红[1] 赵承军[2] 黄洁[1] 孙岳[1] 薛冰[1] 

机构地区：[1]宁夏医科大学医用化学系,宁夏银川750004 [2]宁夏医科大学医学科学技术研究中心,宁夏银川750004

出　　处：《化学研究与应用》2012年第2期192-198,共7页Chemical Research and Application

基　　金：国家自然科学基金项目(20961008);宁夏高等学校科学技术研究重点基金项目(200919341);宁夏自然科学基金项目(NZ09102);教育部科学技术研究重点基金项目(207127)资助

摘　　要：用溴尿嘧啶作内标,制备生物样本、沉降蛋白、酯萃法提取待测组分,建立氟尿嘧啶浓度测定方法,检测"右旋糖酐-磁性层状复合氢氧化物-氟尿嘧啶"(Dextran-Magnetic LDH-Fluorouracil,DMF)给药干预大鼠体内组织中的氟尿嘧啶浓度。结果表明溴尿嘧啶内标HPLC法回收率高(>90%)、精密度好、结果重现性好(RSD<3%)、测试稳定性高;在外加磁场和多次给药干预条件下,测得不同实验组动物组织的药物浓度有统计学意义,满足验证DME磁靶向缓控释性能的分析要求。A HPLC internal standard method was established by deposition protein of biological samples, mixing 5-Bromouracil as internal standard, and extraetion 5-Fluorouraeil with ethyl acetate. The HPLC 5-Bu internal standard method was employed for deter- mination the concentration of 5-Fu in rat tissues after administration with DMF. It was proved that the 5-Bu internal standard methodwas accurate,reproducible and stable, with method recovery more than 90% and reproducible RSD less than 3%. Under external magnetic field and multi-dose administration conditions, the drug concentration of organizations measured in different experimental groups were significant statistically, and the HPLC 5-Bu internal standard method was suit for evaluation the magnetic targeting andcontrolled release performance of DMF model.

关 键 词：“右旋糖酐-磁性层状复合氢氧化物-氟尿嘧啶”转运模型 5-氟尿嘧啶定量检测 HPLC内标法 

分 类 号：O657.7[理学—分析化学]