近红外光谱法测定5-羟基色氨酸的含量  

Near infrared spectroscopy for quantification of 5-hydroxyl tryptophan

作　　者：武佳[1] 杨阳[1] 代涛[1] 汪学楷[1] 林翔[1] 李晖[1] 

机构地区：[1]四川大学化工学院,四川成都610065

出　　处：《化学研究与应用》2012年第2期249-252,共4页Chemical Research and Application

摘　　要：本文利用近红外光谱法建立了5-羟基色氨酸的偏最小二乘(PLS)定量模型。采用相关数法选择波段以及二阶导数、Norris derivative平滑滤波进行数据预处理,所建校正模型的R为0.99907,RMSEC为0.0638,RMSEP为0.0675。经验证模型的预测性能良好,为5-HTP的快速测定提供了一种方法。Near infrared spectroscopy was used to establish a partial least squares （PLS）quantitative model of 5-hydroxyl trypto- phan. By the Correlation coefficient method to select the band and the second derivatives, Norris derivative smoothing filter to pre- treatment the data,R is O. 99907, RMSEC is O, 0638 and RMSEP is O. 0675 in this calibration model. Thought Validating the predic- tion performance is good, a new approach to determination of 5-hydroxyl tryptophan is built.

关 键 词：近红外光谱法 偏最小二乘 5-羟基色氨酸 

分 类 号：O657.33[理学—分析化学]