低聚壳聚糖负载金属卟啉配合物的制备及生物活性研究  

Preparation and biological activitiy of chitooligosaccharide supported porphyrins metallic complexes

作　　者：王荣民[1] 裴菲[1] 何玉凤[1] 谢云涛[1] 宋鹏飞[1] 

机构地区：[1]生态环境相关高分子材料教育部重点实验室,甘肃省高分子材料重点实验室,西北师范大学化学化工学院,甘肃兰州730070

出　　处：《化学研究与应用》2012年第2期278-281,共4页Chemical Research and Application

基　　金：国家自然科学基金(20964002)资助;甘肃省高校基本科研业务费专项资金项目(2010-176)资助

摘　　要：将低聚壳聚糖(COS)分别与不同卟啉金属(MTPPS4,M=Cu,Co,Zn)配合物结合,制备了水溶性低聚壳聚糖负载金属卟啉配合物(COS-MTPPS4),并采用红外光谱和紫外-可见光谱对其结构进行了表征。采用SRB细胞染色法,研究了壳聚糖负载金属卟啉(COS-MTPPS4)对人体肝癌细胞Bel-7402的抗肿瘤细胞活性。结果表明,金属离子配位到卟啉环中,使系列化合物对Bel-7402有较强的抑制生长活性,IC50值均小于100μg/mL,在10～20μg/mL范围内。水溶性低聚壳聚糖金属卟啉配合物作为抗肿瘤药物有很好应用前景。Water soluble metal porphyrin complexes （ MTPPS4, M = Cu, Co, Zn） were conjugated to ehitooligosaecharide （ COS）, and characterized by IR spectra and UV-Vis spectra. Their bioactivities were investigated by SRB method. The results showed that theCOS supported metal porphyrin complexes（ COS-CuTPPS. ）possessed high antitumor activity against liver cancer cell in vitro. The hemi-inhibitory concentration of COS supported metal porphyrin complexes were from 10 to 20μg/mL, which were less than 100μg/mL. The COS supported CuTPPS4 could be applied as a kind of candidate of antitumor drug.

关 键 词：低聚壳聚糖 金属卟啉配合物 SRB法 细胞活性 

分 类 号：O629.12[理学—有机化学]