凝胶渗透色谱-超高效液相色谱法测定鸡蛋中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ残留  

Determination of Sudans Residues in Egg by Gel Permeation Chromatography-Ultra Performance Liquid Chromatography

作　　者：吴宁鹏[1] 彭丽[1] 班付国[1] 贾振民[1] 郭芙蓉[1] 

机构地区：[1]河南省兽药监察所,郑州450008

出　　处：《中国兽药杂志》2012年第2期27-29,32,共4页Chinese Journal of Veterinary Drug

摘　　要：建立了一种简单、实用的鸡蛋中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ残留的凝胶渗透色谱-超高效液相色谱检测方法。样品用乙腈∶丙酮=9∶1(V/V)溶液提取,经凝胶渗透色谱(GPC)净化后,反相液相色谱分离测定。本方法的检测限为20μg/kg,定量限为50μg/kg。四种苏丹红染料在20～2000 ng/mL范围内呈线性相关。在空白鸡蛋中添加50～1000μg/kg浓度下,四种苏丹红的平均回收率为65%～85%,批内变异系数在1.0%～4.8%之间(n=5),批间变异系数在2.1%～5.9%之间(n=3)。结果表明,该法简单、灵敏、特异性强、重现性好,适用于鸡蛋中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ残留的测定。Gel permeation chromatography-ultra-performance liquid chromatography method for simultaneous determination of sudanⅠ、Ⅱ、Ⅲ and Ⅳ was developed.The samples were extracted by acetonitrile： aceton（9∶ 1）,cleaned up by gel permeation chromatography,and determined by ultra-performance liquid chromatography.The limit of detection was 20 μg/kg.The limit of quantification was 50 μg/kg.The calibration curves of the four sudan dyes were linear over the concentration ranges of 20~2000 ng/mL.The average recoveries ranged from 65% to 85%.The inter-run relative standard deviation（RSD） was in the range of 1.0%~4.8%（n=5）,and the between-run relative standard deviation（RSD） was in the range of 2.1%~5.9%（n=3）.Because of its high sensitive,good precision and specificity,the method was found to be suitable for trace analysis of sudans in eggs.

关 键 词：苏丹红 鸡蛋 残留 凝胶渗透色谱 超高效液相色谱 

分 类 号：S859.84[农业科学—临床兽医学]