脂联素球状结构域对3T3-L1脂肪细胞磷酸戊糖途径关键酶表达的影响  

Effect of globular domain of adiponectin on pentose phosphate pathway key enzyme of 3T3 adipocyte

作　　者：王彦[1] 廉如[1] 陈思思[2] 高婷婷[1] 吴彬[3] 陈显久[3] 

机构地区：[1]山西医科大学第一医院内分泌科,山西太原030001 [2]临汾市人民医院内分泌科,山西临汾041000 [3]山西医科大学生物化学与分子生物学教研室,山西太原030001

出　　处：《内科理论与实践》2012年第1期38-41,共4页Journal of Internal Medicine Concepts & Practice

基　　金：山西省科技攻关项目(项目编号:20090321104)

摘　　要：目的:通过探讨脂联素球状结构域(gAd)对3T3-L1脂肪细胞磷酸戊糖途径关键酶表达的影响,进而探讨gAd促进脂肪细胞摄取的葡萄糖是否经磷酸戊糖途径代谢。方法:用gAd干预分化成熟的3T3-L1脂肪细胞,干预结束后测定细胞残液的葡萄糖浓度,并以实时荧光定量PCR(RT-PCR)法检测各组细胞磷酸戊糖途径关键酶葡萄糖-6-磷酸酶(G6PD)转录水平的表达情况,进行统计学分析。结果:各实验组细胞残液中葡萄糖浓度均显著低于对照组(均P<0.01);各实验组G6PD转录水平的表达与对照组相比无差别(F=1.545,P=0.248)。结论:gAd能够促进3T3-L1脂肪细胞摄取胞外葡萄糖,但未导致G6PD转录水平的变化,提示摄取到细胞内的葡萄糖可能不经磷酸戊糖途径进行分解代谢。Objective To investigate the effect of globular domain of adiponectin（gAd） on pentose phosphate pathway key enzyme of 3T3-L1 adipocytes.Methods Matured 3T3-L1 adipocytes were intervened with gAd,and real-time PCR（RT-PCR） was used to determine the transcription level of glucose-6-phosphatase（G6PD）,the key enzyme in pentose phosphate pathway;the changes of transcription level of G6PD were expressed in changes of mRNA levels.All the data were analyzed statistically.Results G6PD transcription level was not different between gAd group and control group.Conclusions gAd promotes the intake of glucose by 3T3-L1 cells,and the catabolism of glucose taken probably was not via the pentose phosphate pathway.

关 键 词：脂联素球状结构域 3T3-L1脂肪细胞 关键酶 磷酸戊糖途径 

分 类 号：R341[医药卫生—基础医学]