不同居群马蔺种质材料同工酶酶谱特征分析  

Isozyme Analysis of Different Chinese Iris Populations

作　　者：李强栋[1,2] 孟林[1] 毛培春[1] 张德罡[2] 

机构地区：[1]北京市农林科学院北京草业与环境研究发展中心,北京100097 [2]甘肃农业大学草业学院,甘肃兰州730070

出　　处：《草地学报》2012年第1期116-124,共9页Acta Agrestia Sinica

基　　金：北京市自然科学基金项目(6102013);北京市农林科学院科技创新能力建设专项(KJCX201101003)资助

摘　　要：采用非连续性聚丙烯酰胺凝胶电泳技术,对我国北方5省区不同居群的23份马蔺(Iris lactea var.chinensis(Fisch.)Koidz.)种质材料进行过氧化物酶(POD)和酯酶(EST)同工酶酶谱特征分析,旨在揭示其遗传多样性,以及不同居群马蔺种质之间的亲缘关系。结果表明:在相对迁移率(Rf)为0.041～0.875的位点处,共有40个位点检测出谱带,其中共有谱带8条,特征谱带15条,说明不同居群间马蔺种质间存在一定同源性,部分居群的马蔺种质会发生遗传变异。来自内蒙临河8号(BJCY-ML013)和吉林长岭19号(BJCY-ML028)之间遗传相似系数最小(0.3726),亲缘关系较远;而新疆伊犁的14,17号和13,18号(BJCY-ML022,BJCY-ML026和BJCY-ML021,BJCY-ML027)之间遗传相似系数均最大(0.9839),亲缘关系较近,其他材料间的相似性系数为0.4049～0.9678。UPG-MA聚类分析结果表明,当相似性系数为0.79时,可将23份野生马蔺种质材料划分为5大类群,分布于相似生态地理环境的马蔺种质材料基本可聚为同一大类,表明23份马蔺种质材料与其分布的生态地理环境存在一定的相关性,但不完全一致。Both peroxidase（POD） and esterase（EST） isozyme patterns of 23 Iris lactea var.chinensis（Fisch.） Koidz.accessions were studied using the vertical polyacrylamide gel electrophoresis in order to demonstrate their blood relationship and genetic diversity.Results indicated that eight general enzyme bands and fifteen specific enzyme bands at forty isozyme loci（Rf from 0.041 to 0.875 loci） were obtained.All tested Chinese Iris had homology,but genetic variation was occurred in some accessions.The lowest genetic similarity（0.3726） was observed between No.8（BJCY-ML013） and No.19（BJCY-ML028） showing their farther genetic relationship.The highest genetic similarity（0.9839） was observed among No.14（BJCY-ML022）,No.17（BJCY-ML026）,No.13（BJCY-ML021） and No.18（BJCY-ML027） indicating their closer genetic relationships.The genetic similarities of other accessions were from 0.4049 to 0.9678 indicating that all tested Chinese Iris had close genetic relationships.Cluster analysis showed that the 23 wild Chinese Iris accessions were classified into 5 groups as the similarity coefficient was 0.79.The germplasm resources from similar geographical habitats were clustered into one group indicating the correlation between all tested 23 Chinese Iris accessions and their geographical habitats.

关 键 词：马蔺 POD EST 亲缘关系 

分 类 号：Q55[生物学—生物化学] Q946.5